Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hicAB/- |
| Location | 9056..9664 | Replicon | chromosome |
| Accession | MK291441 | ||
| Organism | Paracoccus phage vB_PbeS_Pben1 | ||
Toxin (Protein)
| Gene name | hicA | Uniprot ID | - |
| Locus tag | pben1_p26 | Protein ID | AZV00183.1 |
| Coordinates | 9476..9664 (-) | Length | 63 a.a. |
Antitoxin (Protein)
| Gene name | hicB | Uniprot ID | - |
| Locus tag | pben1_p25 | Protein ID | AZV00182.1 |
| Coordinates | 9056..9463 (-) | Length | 136 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| pben1_p11 | 4149..4436 | - | 288 | AZV00168.1 | hypothetical protein | - |
| pben1_p12 | 4503..5354 | - | 852 | AZV00169.1 | hypothetical protein | - |
| pben1_p13 | 5382..5708 | - | 327 | AZV00170.1 | hypothetical protein | - |
| pben1_p14 | 5712..5912 | - | 201 | AZV00171.1 | hypothetical protein | - |
| pben1_p15 | 5909..6106 | - | 198 | AZV00172.1 | hypothetical protein | - |
| pben1_p16 | 6090..6254 | - | 165 | AZV00173.1 | hypothetical protein | - |
| pben1_p17 | 6251..6643 | - | 393 | AZV00174.1 | hypothetical protein | - |
| pben1_p18 | 6640..6906 | - | 267 | AZV00175.1 | hypothetical protein | - |
| pben1_p19 | 7134..7472 | - | 339 | AZV00176.1 | hypothetical protein | - |
| pben1_p20 | 7475..7936 | - | 462 | AZV00177.1 | CI-like repressor | - |
| pben1_p21 | 7935..8183 | + | 249 | AZV00178.1 | Cro-like protein | - |
| pben1_p22 | 8180..8533 | + | 354 | AZV00179.1 | hypothetical protein | - |
| pben1_p23 | 8533..8685 | + | 153 | AZV00180.1 | hypothetical protein | - |
| pben1_p24 | 8682..9038 | + | 357 | AZV00181.1 | hypothetical protein | - |
| pben1_p25 | 9056..9463 | - | 408 | AZV00182.1 | type II toxin-antitoxin HicB family antitoxin | Antitoxin |
| pben1_p26 | 9476..9664 | - | 189 | AZV00183.1 | type II toxin-antitoxin HicA family toxin | Toxin |
| pben1_p27 | 9845..10075 | - | 231 | AZV00184.1 | hypothetical protein | - |
| pben1_p28 | 10058..10291 | + | 234 | AZV00185.1 | hypothetical protein | - |
| pben1_p29 | 10288..10989 | + | 702 | AZV00186.1 | adenine DNA methyltransferase | - |
| pben1_p30 | 10986..11183 | + | 198 | AZV00187.1 | hypothetical protein | - |
| pben1_p31 | 11185..11415 | + | 231 | AZV00188.1 | hypothetical protein | - |
| pben1_p32 | 11399..12019 | + | 621 | AZV00189.1 | putative replication protein | - |
| pben1_p33 | 12266..12835 | + | 570 | AZV00190.1 | hypothetical protein | - |
| pben1_p34 | 12835..13476 | + | 642 | AZV00191.1 | hypothetical protein | - |
| pben1_p35 | 13642..14364 | + | 723 | AZV00192.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 236..38093 | 37857 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 63 a.a. Molecular weight: 14488.19 Da Isoelectric Point: 4.6348
>T10122 AZV00183.1 MK291441:c9664-9476 [Paracoccus phage vB_PbeS_Pben1]
MERNSRKLIKMLEADGWVRVAVKGDHWQFKHPEKPGRVTVPHPNKDIKAGTVMSIYRQAGWR
MERNSRKLIKMLEADGWVRVAVKGDHWQFKHPEKPGRVTVPHPNKDIKAGTVMSIYRQAGWR
Download Length: 189 bp
>T10122 MK291441:c9664-9476 [Paracoccus phage vB_PbeS_Pben1]
ATGGAACGCAACAGCCGGAAGCTGATCAAGATGCTTGAGGCGGACGGCTGGGTCCGGGTCGCGGTCAAGGGCGATCACTG
GCAGTTCAAGCACCCGGAGAAGCCCGGCCGCGTGACCGTTCCGCACCCGAACAAGGACATCAAGGCCGGCACCGTCATGT
CGATCTATCGACAGGCCGGATGGCGCTGA
ATGGAACGCAACAGCCGGAAGCTGATCAAGATGCTTGAGGCGGACGGCTGGGTCCGGGTCGCGGTCAAGGGCGATCACTG
GCAGTTCAAGCACCCGGAGAAGCCCGGCCGCGTGACCGTTCCGCACCCGAACAAGGACATCAAGGCCGGCACCGTCATGT
CGATCTATCGACAGGCCGGATGGCGCTGA
Antitoxin
Download Length: 136 a.a. Molecular weight: 12492.27 Da Isoelectric Point: 11.6753
>AT10122 AZV00182.1 MK291441:c9463-9056 [Paracoccus phage vB_PbeS_Pben1]
MRYVAFLHTDEAGGFGISFPDFPGAISDGDTVEQAIQRGERALAFHVQGMREDGLDVPAPRSVDDILADPDLAEWREGAQ
IAHVALILDRGSPKRVNISLDPGLLDAIDAEAARRGMTRSAFLSSAARAEIHAAH
MRYVAFLHTDEAGGFGISFPDFPGAISDGDTVEQAIQRGERALAFHVQGMREDGLDVPAPRSVDDILADPDLAEWREGAQ
IAHVALILDRGSPKRVNISLDPGLLDAIDAEAARRGMTRSAFLSSAARAEIHAAH
Download Length: 408 bp
>AT10122 MK291441:c9463-9056 [Paracoccus phage vB_PbeS_Pben1]
ATGCGTTACGTTGCTTTCCTGCACACCGATGAAGCCGGCGGCTTCGGCATCAGCTTTCCCGATTTCCCCGGCGCCATCTC
GGACGGCGACACCGTTGAACAGGCCATCCAGCGCGGCGAGCGGGCACTGGCCTTCCATGTCCAGGGCATGCGCGAGGACG
GCCTCGACGTCCCGGCCCCCCGCAGTGTAGACGACATCCTGGCCGATCCCGACCTTGCCGAATGGCGCGAGGGCGCACAG
ATCGCCCATGTGGCGCTGATTCTGGATCGCGGATCGCCCAAGCGGGTGAACATTTCCCTGGACCCTGGCCTCCTGGACGC
CATCGACGCCGAGGCGGCGCGGCGCGGCATGACGCGCAGCGCGTTCCTGTCCTCGGCCGCGCGCGCGGAAATCCATGCGG
CGCACTGA
ATGCGTTACGTTGCTTTCCTGCACACCGATGAAGCCGGCGGCTTCGGCATCAGCTTTCCCGATTTCCCCGGCGCCATCTC
GGACGGCGACACCGTTGAACAGGCCATCCAGCGCGGCGAGCGGGCACTGGCCTTCCATGTCCAGGGCATGCGCGAGGACG
GCCTCGACGTCCCGGCCCCCCGCAGTGTAGACGACATCCTGGCCGATCCCGACCTTGCCGAATGGCGCGAGGGCGCACAG
ATCGCCCATGTGGCGCTGATTCTGGATCGCGGATCGCCCAAGCGGGTGAACATTTCCCTGGACCCTGGCCTCCTGGACGC
CATCGACGCCGAGGCGGCGCGGCGCGGCATGACGCGCAGCGCGTTCCTGTCCTCGGCCGCGCGCGCGGAAATCCATGCGG
CGCACTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT875 | Sinorhizobium meliloti 1021 |
38.806 |
99.259 |
0.385 |
| AT6107 | Burkholderia pseudomallei K96243 |
35.385 |
96.296 |
0.341 |
| AT3593 | Acinetobacter baumannii ATCC 17978 |
33.333 |
91.111 |
0.304 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Przemyslaw Decewicz et al. (2019) Characterization of the virome of Paracoccus spp. (Alphaproteobacteria) by combined in silico and in vivo approaches. Scientific Reports 9(1):7899. [PubMed:31133656]