Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hicAB/HicB-HicA |
| Location | 2199475..2200129 | Replicon | chromosome |
| Accession | NC_003047 | ||
| Organism | Sinorhizobium meliloti 1021 | ||
Toxin (Protein)
| Gene name | hicA | Uniprot ID | Q92K60 |
| Locus tag | SMc04441 | Protein ID | 15965792 |
| Coordinates | 2199475..2199663 (+) | Length | 63 a.a. |
Antitoxin (Protein)
| Gene name | hicB | Uniprot ID | Q92NX0 |
| Locus tag | SMc04269 | Protein ID | 15965793 |
| Coordinates | 2199668..2200129 (+) | Length | 154 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SM_RS10445 (SMc04208) | 2196041..2197351 | + | 1311 | WP_010969656.1 | HlyD family type I secretion periplasmic adaptor subunit | - |
| SM_RS10450 (SMc04209) | 2197376..2198347 | - | 972 | WP_017265202.1 | glycosyltransferase family 2 protein | - |
| SM_RS10455 (SMc04267) | 2198528..2199298 | - | 771 | WP_010969658.1 | hypothetical protein | - |
| SM_RS10460 (SMc04441) | 2199475..2199663 | + | 189 | WP_003530010.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
| SM_RS10465 (SMc04269) | 2199668..2200129 | + | 462 | WP_010969659.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
| SM_RS10475 (SMc04271) | 2200448..2201365 | - | 918 | WP_003530005.1 | PhzF family phenazine biosynthesis protein | - |
| SM_RS10480 (SMc04272) | 2201542..2202291 | - | 750 | WP_010969660.1 | NUDIX hydrolase | - |
| SM_RS10485 (SMc04274) | 2202294..2203400 | - | 1107 | WP_010969661.1 | endonuclease | - |
| SM_RS10490 (SMc04276) | 2203551..2203994 | - | 444 | WP_003529999.1 | TIGR02594 family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 63 a.a. Molecular weight: 6971.15 Da Isoelectric Point: 11.0889
>T875 15965792 NC_003047:2199475-2199663 [Sinorhizobium meliloti 1021]
MKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR
MKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR
Download Length: 189 bp
>T875 NC_003047:2199475-2199663 [Sinorhizobium meliloti 1021]
ATGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA
ATGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA
Antitoxin
Download Length: 154 a.a. Molecular weight: 16293.17 Da Isoelectric Point: 4.3990
>AT875 15965793 NC_003047:2199668-2200129 [Sinorhizobium meliloti 1021]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV
Download Length: 462 bp
>AT875 NC_003047:2199668-2200129 [Sinorhizobium meliloti 1021]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAGGTTGTCATGTCCGACGCCGAGAACAGGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCCTATGCGGAATCCCGCTTGTCGGCTTTGGGCACTTCCAGCAAAAATGTGTAA
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAGGTTGTCATGTCCGACGCCGAGAACAGGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCCTATGCGGAATCCCGCTTGTCGGCTTTGGGCACTTCCAGCAAAAATGTGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T3593 | Acinetobacter baumannii ATCC 17978 |
62.745 |
98.077 |
0.615 |
| T6107 | Burkholderia pseudomallei K96243 |
61.017 |
100 |
0.61 |
| T10122 | Paracoccus phage vB_PbeS_Pben1 |
38.806 |
99.259 |
0.385 |
| T10168 | Streptococcus pneumoniae TIGR4 |
32.258 |
100 |
0.339 |
| T6283 | Pseudomonas aeruginosa PA1 |
31.667 |
100 |
0.317 |
| T1325 | Thermotoga maritima MSB8 |
34.545 |
88.71 |
0.306 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6107 | Burkholderia pseudomallei K96243 |
40 |
94.203 |
0.377 |
Multiple sequence alignment
References
(1) Manon Thomet et al. (2019) Characterization of HicAB toxin-antitoxin module of Sinorhizobium meliloti. BMC Microbiology 19(1):10. [PubMed:30630415]