Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hicAB/HicA(toxin) |
| Location | 1332765..1333213 | Replicon | chromosome |
| Accession | NC_000853 | ||
| Organism | Thermotoga maritima MSB8 | ||
Toxin (Protein)
| Gene name | hicA | Uniprot ID | Q9X138 |
| Locus tag | TM_RS06645 | Protein ID | WP_004079902.1 |
| Coordinates | 1332765..1332998 (-) | Length | 78 a.a. |
Antitoxin (Protein)
| Gene name | hicA | Uniprot ID | G4FE38 |
| Locus tag | TM_RS06650 | Protein ID | WP_004079900.1 |
| Coordinates | 1332995..1333213 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| TM_RS06615 (TM1304) | 1328283..1329089 | + | 807 | WP_004079915.1 | ABC transporter permease | - |
| TM_RS06620 (TM1306) | 1329307..1329741 | + | 435 | WP_004079914.1 | ABC transporter permease | - |
| TM_RS06625 | 1329745..1330515 | + | 771 | Protein_1284 | ABC transporter permease | - |
| TM_RS06630 (TM1310) | 1330578..1332320 | + | 1743 | WP_004079909.1 | ABC transporter ATP-binding protein/permease | - |
| TM_RS06635 | 1332391..1332540 | - | 150 | WP_081434644.1 | type II toxin-antitoxin system HicA family toxin | - |
| TM_RS06640 (TM1311) | 1332530..1332742 | - | 213 | WP_004079908.1 | hypothetical protein | - |
| TM_RS06645 (TM1312) | 1332765..1332998 | - | 234 | WP_004079902.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
| TM_RS06650 (TM1313) | 1332995..1333213 | - | 219 | WP_004079900.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
| TM_RS06655 (TM1314) | 1333310..1334917 | - | 1608 | WP_010865307.1 | helix-turn-helix domain-containing protein | - |
| TM_RS06660 (TM1315) | 1335113..1335277 | + | 165 | WP_004083364.1 | hypothetical protein | - |
| TM_RS06665 (TM1316) | 1335354..1335449 | + | 96 | WP_010865309.1 | Cys-rich RiPP peptide | - |
| TM_RS06670 (TM1317) | 1335551..1336942 | + | 1392 | WP_004083362.1 | radical SAM protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Genomic island | - | - | 1325051..1336942 | 11891 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 78 a.a. Molecular weight: 8983.60 Da Isoelectric Point: 10.4691
>T1325 WP_004079902.1 NC_000853:c1332998-1332765 [Thermotoga maritima MSB8]
MSYLPIVDPKTMEKVLLKLGFQRVRQKGSHVFYRHSNGKYTTIPFHAKETCPSHLIRKIIREAGISVEEFKKVLENL
MSYLPIVDPKTMEKVLLKLGFQRVRQKGSHVFYRHSNGKYTTIPFHAKETCPSHLIRKIIREAGISVEEFKKVLENL
Download Length: 234 bp
>T1325 NC_000853:c1332998-1332765 [Thermotoga maritima MSB8]
TTGAGTTATCTTCCAATAGTTGATCCAAAAACTATGGAAAAGGTTTTACTGAAACTTGGATTTCAACGTGTTCGCCAAAA
AGGAAGTCATGTGTTCTACAGGCACAGCAATGGAAAGTATACGACCATCCCATTTCATGCAAAAGAGACTTGCCCAAGTC
ACTTAATAAGAAAAATCATCCGTGAAGCGGGTATATCAGTGGAGGAATTCAAGAAAGTACTTGAGAATCTGTGA
TTGAGTTATCTTCCAATAGTTGATCCAAAAACTATGGAAAAGGTTTTACTGAAACTTGGATTTCAACGTGTTCGCCAAAA
AGGAAGTCATGTGTTCTACAGGCACAGCAATGGAAAGTATACGACCATCCCATTTCATGCAAAAGAGACTTGCCCAAGTC
ACTTAATAAGAAAAATCATCCGTGAAGCGGGTATATCAGTGGAGGAATTCAAGAAAGTACTTGAGAATCTGTGA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8186.38 Da Isoelectric Point: 4.0891
>AT1325 WP_004079900.1 NC_000853:c1333213-1332995 [Thermotoga maritima MSB8]
VRKIFTAIIEYDPEKKQYVGMVPDVPGVHTVGSSLEEVRRNLKEVLELVLEEAGDEINLQEFVALEMIEVET
VRKIFTAIIEYDPEKKQYVGMVPDVPGVHTVGSSLEEVRRNLKEVLELVLEEAGDEINLQEFVALEMIEVET
Download Length: 219 bp
>AT1325 NC_000853:c1333213-1332995 [Thermotoga maritima MSB8]
GTGCGTAAAATTTTCACAGCTATCATTGAATACGATCCTGAAAAAAAGCAATATGTTGGAATGGTTCCAGATGTTCCAGG
TGTACATACTGTAGGTAGTTCCTTAGAAGAAGTGAGAAGGAATTTAAAAGAGGTGCTTGAACTCGTATTGGAGGAAGCAG
GAGATGAAATAAATCTCCAGGAATTCGTGGCTCTTGAGATGATAGAGGTGGAAACTTGA
GTGCGTAAAATTTTCACAGCTATCATTGAATACGATCCTGAAAAAAAGCAATATGTTGGAATGGTTCCAGATGTTCCAGG
TGTACATACTGTAGGTAGTTCCTTAGAAGAAGTGAGAAGGAATTTAAAAGAGGTGCTTGAACTCGTATTGGAGGAAGCAG
GAGATGAAATAAATCTCCAGGAATTCGTGGCTCTTGAGATGATAGAGGTGGAAACTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T3593 | Acinetobacter baumannii ATCC 17978 |
35.417 |
92.308 |
0.327 |
| T875 | Sinorhizobium meliloti 1021 |
34.545 |
88.71 |
0.306 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Andrew D Frock et al. (2013) Stationary phase and nutrient levels trigger transcription of a genomic locus containing a novel peptide (TM1316) in the hyperthermophilic bacterium Thermotoga maritima. Applied And Environmental Microbiology 79(21):6637-46. [PubMed:23974142]