Detailed information of TA system TA006283

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1462677..1463325
• Replicon: chromosome
• Accession: NC_022808
• Organism: Pseudomonas aeruginosa PA1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8G3IXE4
• Locus tag: PA1S_RS06915
• Protein ID: WP_003098540.1
• Coordinates: 1463143..1463325 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: PA1S_RS31585
• Protein ID: WP_228478067.1
• Coordinates: 1462377..1463096 (-)
• Length: 240 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA1S_RS06875 (PA1S_06885)	1458268..1458972	+	705	WP_079279906.1	phage regulatory protein/antirepressor Ant	-
PA1S_RS06880 (PA1S_06890)	1459037..1459798	+	762	WP_043486980.1	helix-turn-helix domain-containing protein	-
PA1S_RS06885 (PA1S_06895)	1459795..1460478	+	684	WP_043486977.1	hypothetical protein	-
PA1S_RS06890 (PA1S_06900)	1460475..1460681	+	207	WP_003159465.1	hypothetical protein	-
PA1S_RS06895 (PA1S_06905)	1460678..1461259	+	582	WP_021205616.1	recombination protein NinG	-
PA1S_RS06900 (PA1S_06910)	1461256..1461552	+	297	WP_043486973.1	hypothetical protein	-
PA1S_RS06905 (PA1S_06915)	1461590..1462264	+	675	WP_043486972.1	hypothetical protein	-
PA1S_RS31585	1462377..1463096	-	720	WP_228478067.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PA1S_RS06915 (PA1S_06925)	1463143..1463325	-	183	WP_003098540.1	type II toxin-antitoxin system HicA family toxin	Toxin
PA1S_RS30320	1463541..1464062	+	522	WP_158309806.1	GspH/FimT family pseudopilin	-
PA1S_RS06920 (PA1S_06930)	1464246..1464560	+	315	WP_023517910.1	phage holin, lambda family	-
PA1S_RS06925 (PA1S_06935)	1464560..1465177	+	618	WP_023517909.1	glycoside hydrolase family 19 protein	-
PA1S_RS06930 (PA1S_06940)	1465195..1465626	+	432	WP_194736254.1	hypothetical protein	-
PA1S_RS30325 (PA1S_06945)	1465763..1466341	+	579	WP_079279905.1	HNH endonuclease	-
PA1S_RS06935 (PA1S_06950)	1466248..1466730	+	483	WP_033940323.1	phage terminase small subunit P27 family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1445400..1485712	40312

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6746.86 Da
• Isoelectric Point: 11.0775

>T6283 WP_003098540.1 NC_022808:c1463325-1463143 [Pseudomonas aeruginosa PA1]
MKFSEFRRWLKAQGVTFEAGKGSHFKITAPNGKQTTFADHGAKEMPEPTRKAIIKQLGLK

Nucleotide

• Length: 183 bp

>T6283 NC_022808:c1463325-1463143 [Pseudomonas aeruginosa PA1]
ATGAAGTTCAGCGAATTCAGACGATGGTTGAAGGCCCAAGGGGTGACCTTCGAAGCCGGCAAGGGAAGCCACTTCAAGAT
CACCGCCCCGAACGGCAAACAGACCACCTTCGCGGACCACGGAGCTAAGGAAATGCCAGAACCGACCCGCAAGGCGATCA
TCAAGCAACTGGGGCTCAAATGA

Antitoxin

Protein

• Length: 240 a.a.
• Molecular weight: 26723.27 Da
• Isoelectric Point: 5.1740

>AT6283 WP_228478067.1 NC_022808:c1463096-1462377 [Pseudomonas aeruginosa PA1]
MYDYAIRFEQDDSAPGVAVFCRDLPELNSYGDDKTHAISEALDAIGTTLSLYIDARKSIPEATPPEEGEHVVHLPAVTVA
KIALWNEMMKRGMRKSDLCRLLGIAQTQGDRLVDFLHHTKMDAVESALLALGIRLRQSVEVRFDEVKLYLERDTTVVLSE
AWCELSASCKDEAGNYRGWLSLPSQTSPQRQTIKGIEAHRLASHCHLSISVDDDRPGEAPFESQGRIHLPVTFYSVEVK

Nucleotide

• Length: 720 bp

>AT6283 NC_022808:c1463096-1462377 [Pseudomonas aeruginosa PA1]
ATGTACGACTATGCAATCCGTTTCGAACAGGACGATAGCGCTCCTGGCGTTGCCGTTTTCTGCAGAGACTTGCCGGAACT
GAACAGCTACGGCGACGATAAGACCCATGCAATCAGCGAGGCGCTTGACGCCATCGGGACGACCCTGTCGCTCTACATTG
ACGCGCGAAAATCGATCCCGGAAGCCACTCCACCAGAGGAGGGCGAGCATGTCGTCCACCTGCCAGCCGTCACGGTGGCG
AAGATCGCGCTGTGGAACGAGATGATGAAGCGCGGAATGCGCAAGTCCGACCTGTGCAGGCTCCTGGGCATCGCCCAAAC
CCAGGGCGATCGCCTGGTGGACTTCTTGCACCACACAAAAATGGACGCCGTGGAGAGCGCCCTACTGGCACTTGGCATCC
GGCTTCGTCAATCAGTTGAGGTCAGGTTTGACGAGGTGAAACTTTACCTCGAGCGTGACACTACGGTGGTCTTATCAGAG
GCATGGTGCGAACTGAGCGCATCCTGCAAAGACGAAGCTGGAAATTATCGTGGATGGTTAAGCCTTCCAAGCCAGACATC
CCCCCAGCGGCAAACAATCAAAGGGATTGAGGCTCATCGACTCGCCTCCCACTGTCACCTCAGCATATCGGTAGATGATG
ATAGGCCTGGCGAAGCACCTTTCGAATCGCAGGGGCGAATTCACTTGCCAGTAACGTTCTACTCAGTAGAAGTAAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T10189	Escherichia coli strain DH5alpha	47.458	100	0.483
T1039	Escherichia coli str. K-12 substr. MG1655	47.458	100	0.483
T10168	Streptococcus pneumoniae TIGR4	37.255	86.441	0.322
T875	Sinorhizobium meliloti 1021	31.667	100	0.317
T6107	Burkholderia pseudomallei K96243	30.508	100	0.305

Multiple sequence alignment

References

(1) Gang Li et al. (2016) Identification and Characterization of the HicAB Toxin-Antitoxin System in the Opportunistic Pathogen Pseudomonas aeruginosa. Toxins 8(4):113. [PubMed:27104566]


 experimental literature