Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1703683..1704351 Replicon chromosome
Accession NC_003028
Organism Streptococcus pneumoniae TIGR4

Toxin (Protein)


Gene name hicA Uniprot ID A0A0B7M7X4
Locus tag SP_RS08870 Protein ID WP_001132284.1
Coordinates 1704172..1704351 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID G0IC64
Locus tag SP_RS08865 Protein ID WP_000961810.1
Coordinates 1703683..1704135 (-) Length 151 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SP_RS08840 (SP_1781) 1699575..1700318 - 744 WP_001188203.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
SP_RS08845 (SP_1782) 1700320..1701270 - 951 WP_000451153.1 50S ribosomal protein L11 methyltransferase -
SP_RS08850 (SP_1783) 1701406..1701834 - 429 WP_000418173.1 NUDIX hydrolase -
SP_RS08855 (SP_1784) 1701815..1702903 - 1089 WP_000719713.1 M50 family metallopeptidase -
SP_RS08860 (SP_1785) 1702922..1703392 - 471 WP_000257087.1 DUF3013 family protein -
SP_RS08865 (SP_1786) 1703683..1704135 - 453 WP_000961810.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
SP_RS08870 (SP_1787) 1704172..1704351 - 180 WP_001132284.1 type II toxin-antitoxin system HicA family toxin Toxin
SP_RS08875 1704488..1704667 - 180 WP_001048906.1 hypothetical protein -
SP_RS08880 (SP_1789) 1704832..1705047 - 216 WP_075329187.1 hypothetical protein -
SP_RS08885 (SP_1790) 1705345..1706616 + 1272 WP_001113214.1 replication-associated recombination protein A -
SP_RS13185 (SP_1791) 1707036..1707152 - 117 WP_001855795.1 hypothetical protein -
SP_RS08895 (SP_1792) 1707173..1708429 - 1257 WP_000436400.1 ISL3 family transposase -
SP_RS08900 (SP_1793) 1708735..1709310 - 576 WP_000810872.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1707173..1708429 1256


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(17-114)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6713.95 Da        Isoelectric Point: 11.0174

>T10168 WP_001132284.1 NC_003028:c1704351-1704172 [Streptococcus pneumoniae TIGR4]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGELNKYTERGIRKQAGL

Download         Length: 180 bp

>T10168 NC_003028:c1704351-1704172 [Streptococcus pneumoniae TIGR4]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAAAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin


Download         Length: 151 a.a.        Molecular weight: 16659.46 Da        Isoelectric Point: 3.7220

>AT10168 WP_000961810.1 NC_003028:c1704135-1703683 [Streptococcus pneumoniae TIGR4]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Download         Length: 453 bp

>AT10168 NC_003028:c1704135-1703683 [Streptococcus pneumoniae TIGR4]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGGCTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T875 Sinorhizobium meliloti 1021

32.258

100

0.339
T6283 Pseudomonas aeruginosa PA1

37.255

86.441

0.322
T6107 Burkholderia pseudomallei K96243

30

100

0.305

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B7M7X4

Antitoxin

Source ID Structure
PDB 5YRZ
AlphaFold DB G0IC64

References

(1) Do-Hee Kim et al. (2018) Functional insights into the Streptococcus pneumoniae HicBA toxin-antitoxin system based on a structural study. Nucleic Acids Research 46(12):6371-6386. [PubMed:29878152] 3D_structure


3D_structure protein structure literature