Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1696492..1697160 Replicon chromosome
Accession NZ_CP089948
Organism Streptococcus pneumoniae TIGR4

Toxin (Protein)


Gene name hicA Uniprot ID A0A0B7M7X4
Locus tag LXA46_RS08385 Protein ID WP_001132284.1
Coordinates 1696981..1697160 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID G0IC64
Locus tag LXA46_RS08380 Protein ID WP_000961810.1
Coordinates 1696492..1696944 (-) Length 151 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXA46_RS08355 (LXA46_08345) 1692384..1693127 - 744 WP_001188203.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
LXA46_RS08360 (LXA46_08350) 1693129..1694079 - 951 WP_000451153.1 50S ribosomal protein L11 methyltransferase -
LXA46_RS08365 (LXA46_08355) 1694215..1694643 - 429 WP_000418173.1 NUDIX hydrolase -
LXA46_RS08370 (LXA46_08360) 1694624..1695712 - 1089 WP_000719713.1 site-2 protease family protein -
LXA46_RS08375 (LXA46_08365) 1695731..1696201 - 471 WP_000257087.1 DUF3013 family protein -
LXA46_RS08380 (LXA46_08370) 1696492..1696944 - 453 WP_000961810.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LXA46_RS08385 (LXA46_08375) 1696981..1697160 - 180 WP_001132284.1 type II toxin-antitoxin system HicA family toxin Toxin
LXA46_RS08390 (LXA46_08380) 1697297..1697476 - 180 WP_001048906.1 hypothetical protein -
LXA46_RS08395 (LXA46_08385) 1697641..1697862 - 222 WP_000425689.1 hypothetical protein -
LXA46_RS08400 (LXA46_08390) 1698154..1699425 + 1272 WP_001113214.1 replication-associated recombination protein A -
LXA46_RS08410 (LXA46_08400) 1699982..1701238 - 1257 WP_000436400.1 ISL3 family transposase -
LXA46_RS08415 (LXA46_08405) 1701544..1702119 - 576 WP_000810872.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1699982..1701238 1256


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(17-114)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6713.95 Da        Isoelectric Point: 11.0174

>T229790 WP_001132284.1 NZ_CP089948:c1697160-1696981 [Streptococcus pneumoniae TIGR4]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGELNKYTERGIRKQAGL

Download         Length: 180 bp

>T229790 NZ_CP124517:c3011417-3011310 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 151 a.a.        Molecular weight: 16659.46 Da        Isoelectric Point: 3.7220

>AT229790 WP_000961810.1 NZ_CP089948:c1696944-1696492 [Streptococcus pneumoniae TIGR4]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Download         Length: 453 bp

>AT229790 NZ_CP124517:3011466-3011531 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B7M7X4

Antitoxin

Source ID Structure
PDB 5YRZ
AlphaFold DB G0IC64

References