Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hicAB/HicB-HicA |
| Location | 1507310..1507948 | Replicon | chromosome |
| Accession | NC_000913 | ||
| Organism | Escherichia coli str. K-12 substr. MG1655 | ||
Toxin (Protein)
| Gene name | hicA | Uniprot ID | P76106 |
| Locus tag | b4532 | Protein ID | NP_415954.2 |
| Coordinates | 1509286..1509462 (+) | Length | 59 a.a. |
Antitoxin (Protein)
| Gene name | hicB | Uniprot ID | P67697 |
| Locus tag | b1438 | Protein ID | NP_415955.2 |
| Coordinates | 1509508..1509924 (+) | Length | 139 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| b1431 | 1502457..1503125 | + | 669 | NP_415948.1 | DUF3313 domain-containing lipoprotein YdcL | - |
| b1432 | 1503657..1504865 | + | 1209 | NP_415949.3 | putative RNA-guided DNA endonuclease InsQ | - |
| b1433 | 1504905..1506080 | - | 1176 | NP_415950.4 | putative transport protein YdcO | - |
| b1434 | 1506172..1506708 | + | 537 | NP_415951.1 | DNA-binding transcriptional dual regulator SutR | - |
| b1435 | 1506781..1508742 | + | 1962 | NP_415952.2 | 23S rRNA 5-hydroxycytidine C2501 synthase | - |
| b1436 | 1508834..1509064 | - | 231 | NP_415953.1 | DUF2554 domain-containing protein YncJ | - |
| b4532 | 1509286..1509462 | + | 177 | NP_415954.2 | mRNA interferase toxin HicA | Toxin |
| b1438 | 1509508..1509924 | + | 417 | NP_415955.2 | antitoxin of the HicA-HicB toxin-antitoxin system/ DNA-binding transcriptional repressor HicB | Antitoxin |
| b1439 | 1510003..1511409 | + | 1407 | NP_415956.1 | fused putative DNA-binding transcriptional regulator/putative aminotransferase YdcR | - |
| b1440 | 1511654..1512799 | + | 1146 | NP_415957.1 | putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 59 a.a. Molecular weight: 6749.83 Da Isoelectric Point: 11.2298
>T1039 NP_415954.2 NC_000913:1509286-1509462 [Escherichia coli str. K-12 substr. MG1655]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS
Download Length: 177 bp
>T1039 NC_000913:1509286-1509462 [Escherichia coli str. K-12 substr. MG1655]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA
Antitoxin
Download Length: 139 a.a. Molecular weight: 15246.63 Da Isoelectric Point: 4.7386
>AT1039 NP_415955.2 NC_000913:1509508-1509924 [Escherichia coli str. K-12 substr. MG1655]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV
Download Length: 417 bp
>AT1039 NC_000913:1509508-1509924 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10189 | Escherichia coli strain DH5alpha |
100 |
100 |
1 |
| T6283 | Pseudomonas aeruginosa PA1 |
47.458 |
100 |
0.483 |
| T3593 | Acinetobacter baumannii ATCC 17978 |
31.481 |
100 |
0.327 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10189 | Escherichia coli strain DH5alpha |
100 |
100 |
1 |
Multiple sequence alignment
References
(1) Melek Cemre Manav et al. (2019) The E. coli HicB Antitoxin Contains a Structurally Stable Helix-Turn-Helix DNA Binding Domain. Structure (London, England : 1993) 27(11):1675-1685.e3. [PubMed:31495532]
(2) Kathryn J Turnbull et al. (2017) HicA toxin of Escherichia coli derepresses hicAB transcription to selectively produce HicB antitoxin. Molecular Microbiology 104(5):781-792. [PubMed:28266056]
(3) Tiffany M Halvorsen et al. (2022) Comparison of Kill Switch Toxins in Plant-Beneficial Pseudomonas fluorescens Reveals Drivers of Lethality, Stability, and Escape. ACS Synthetic Biology 11(11):3785-3796. [PubMed:36346907]
(4) Mikkel G Jørgensen et al. (2009) HicA of Escherichia coli defines a novel family of translation-independent mRNA interferases in bacteria and archaea. Journal of Bacteriology 191(4):1191-9. [PubMed:19060138]