10189

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 366728..367366 Replicon chromosome
Accession NZ_CP025520
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CDN75_RS02075 Protein ID WP_000813794.1
Coordinates 366728..366904 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CDN75_RS02080 Protein ID WP_001270286.1
Coordinates 366950..367366 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDN75_RS02055 362347..363561 - 1215 WP_001326689.1 BenE family transporter YdcO -
CDN75_RS02060 363614..364150 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
CDN75_RS02065 364223..366184 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CDN75_RS02070 366276..366506 - 231 WP_000494244.1 YncJ family protein -
CDN75_RS02075 366728..366904 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CDN75_RS02080 366950..367366 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CDN75_RS02085 367445..368851 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
CDN75_RS02090 369096..370241 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
CDN75_RS02095 370259..371272 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
CDN75_RS02100 371273..372214 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T10189 WP_000813794.1 NZ_CP025520:366728-366904 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T10189 NZ_CP025520:366728-366904 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT10189 WP_001270286.1 NZ_CP025520:366950-367366 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT10189 NZ_CP025520:366950-367366 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T1039 Escherichia coli str. K-12 substr. MG1655

100

100

1
T6283 Pseudomonas aeruginosa PA1

47.458

100

0.483
T3593 Acinetobacter baumannii ATCC 17978

31.481

100

0.327

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT1039 Escherichia coli str. K-12 substr. MG1655

100

100

1

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References

(1) Jingsi Yang et al. (2017) HicAB toxin-antitoxin complex from Escherichia coli: expression and crystallization. Acta Crystallographica. Section F, Structural Biology Communications 73(Pt 9):505-510. [PubMed:28876228] 3D_structure


3D_structure protein structure literature