47908

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 1289260..1289797 Replicon chromosome
Accession NZ_CP008910
Organism Burkholderia pseudomallei MSHR5848

Toxin (Protein)


Gene name brnT Uniprot ID Q2T6G2
Locus tag DP65_RS23465 Protein ID WP_004549731.1
Coordinates 1289528..1289797 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag DP65_RS23460 Protein ID WP_004525420.1
Coordinates 1289260..1289544 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DP65_RS23440 1284368..1285630 - 1263 WP_004530738.1 c-type cytochrome -
DP65_RS23445 1285789..1287249 - 1461 WP_004547073.1 metallophosphoesterase -
DP65_RS36785 1287496..1287612 + 117 Protein_1009 IS3 family transposase -
DP65_RS36790 1287838..1288266 - 429 Protein_1010 IS110 family transposase -
DP65_RS23450 1288229..1288612 - 384 Protein_1011 DDE-type integrase/transposase/recombinase -
DP65_RS23460 1289260..1289544 - 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
DP65_RS23465 1289528..1289797 - 270 WP_004549731.1 BrnT family toxin Toxin
DP65_RS36805 1290350..1290625 + 276 WP_038726742.1 hypothetical protein -
DP65_RS23480 1290902..1291123 + 222 WP_024430861.1 hypothetical protein -
DP65_RS40990 1291171..1291389 + 219 WP_004552392.1 hypothetical protein -
DP65_RS36810 1291575..1292230 - 656 Protein_1017 hypothetical protein -
DP65_RS41495 1292476..1292625 + 150 WP_043276559.1 hypothetical protein -
DP65_RS41500 1292622..1293395 + 774 Protein_1019 prohibitin family protein -
DP65_RS41505 1293393..1293821 + 429 Protein_1020 DDE-type integrase/transposase/recombinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1288229..1293821 5592
flank IS/Tn - - 1288229..1288525 296


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10157.46 Da        Isoelectric Point: 5.6831

>T47908 WP_004549731.1 NZ_CP008910:c1289797-1289528 [Burkholderia pseudomallei MSHR5848]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T47908 NZ_CP008910:c1289797-1289528 [Burkholderia pseudomallei MSHR5848]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT47908 WP_004525420.1 NZ_CP008910:c1289544-1289260 [Burkholderia pseudomallei MSHR5848]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT47908 NZ_CP008910:c1289544-1289260 [Burkholderia pseudomallei MSHR5848]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q2T6G2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References