21103

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 539214..539655 Replicon chromosome
Accession NC_006351
Organism Burkholderia pseudomallei K96243

Toxin (Protein)


Gene name brnT Uniprot ID -
Locus tag BPS_RS20840 Protein ID WP_009939402.1
Coordinates 539482..539655 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag BPS_RS20835 Protein ID WP_004525420.1
Coordinates 539214..539498 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BPS_RS20805 (BPSS0388) 534439..535173 - 735 WP_004552313.1 VRR-NUC domain-containing protein -
BPS_RS20810 (BPSS0389) 535170..535697 - 528 WP_004552312.1 PAAR domain-containing protein -
BPS_RS20815 (BPSS0390) 535977..536156 + 180 WP_009893919.1 type II toxin-antitoxin system HicA family toxin -
BPS_RS20820 (BPSS0391) 536201..536617 + 417 WP_004552310.1 type II toxin-antitoxin system HicB family antitoxin -
BPS_RS20825 (BPSS0391A) 537046..537543 - 498 WP_004552308.1 hypothetical protein -
BPS_RS20830 (BPSS0392) 538161..538568 - 408 Protein_443 DDE-type integrase/transposase/recombinase -
BPS_RS20835 (BPSS0394) 539214..539498 - 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
BPS_RS20840 (BPSS0395) 539482..539655 - 174 WP_009939402.1 BrnT family toxin Toxin
BPS_RS20845 (BPSS0396) 540323..540598 + 276 WP_004552388.1 bacteriophage protein Gp49 -
BPS_RS20850 (BPSS0397) 540608..540739 + 132 WP_009923452.1 bacteriophage protein Gp48 -
BPS_RS20855 (BPSS0398) 540875..541096 + 222 WP_004552390.1 hypothetical protein -
BPS_RS20860 541063..541362 + 300 WP_160469016.1 hypothetical protein -
BPS_RS20865 (BPSS0400) 541537..542127 - 591 WP_038717720.1 hypothetical protein -
BPS_RS20870 (BPSS0401) 542377..542526 + 150 WP_004537646.1 bacteriophage protein Gp44 -
BPS_RS20875 (BPSS0402) 542523..543296 + 774 Protein_452 prohibitin family protein -
BPS_RS32135 543294..543698 + 405 Protein_453 DDE-type integrase/transposase/recombinase -
BPS_RS20880 (BPSS0402A) 543661..544089 + 429 Protein_454 IS110 family transposase -
BPS_RS20885 (BPSS0402B) 544316..544432 - 117 Protein_455 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 516820..546139 29319
flank IS/Tn - - 538161..538481 320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(4-49)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6691.59 Da        Isoelectric Point: 7.9950

>T21103 WP_009939402.1 NC_006351:c539655-539482 [Burkholderia pseudomallei K96243]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Download         Length: 174 bp

>T21103 NC_006351:c539655-539482 [Burkholderia pseudomallei K96243]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT21103 WP_004525420.1 NC_006351:c539498-539214 [Burkholderia pseudomallei K96243]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT21103 NC_006351:c539498-539214 [Burkholderia pseudomallei K96243]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGTTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References