49223

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2332934..2333574 Replicon chromosome
Accession NZ_CP009537
Organism Burkholderia pseudomallei K96243

Toxin (Protein)


Gene name hicA Uniprot ID Q2T3Q6
Locus tag AQ15_RS09590 Protein ID WP_009893919.1
Coordinates 2332934..2333113 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q63NA5
Locus tag AQ15_RS09595 Protein ID WP_004552310.1
Coordinates 2333158..2333574 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AQ15_RS09570 2329625..2330200 + 576 WP_004552315.1 recombinase family protein -
AQ15_RS09575 2330271..2331386 - 1116 WP_004552314.1 DUF3396 domain-containing protein -
AQ15_RS09580 2331396..2332130 - 735 WP_004552313.1 VRR-NUC domain-containing protein -
AQ15_RS09585 2332127..2332654 - 528 WP_004552312.1 PAAR domain-containing protein -
AQ15_RS09590 2332934..2333113 + 180 WP_009893919.1 type II toxin-antitoxin system HicA family toxin Toxin
AQ15_RS09595 2333158..2333574 + 417 WP_004552310.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AQ15_RS09600 2334003..2334500 - 498 WP_004552308.1 hypothetical protein -
AQ15_RS34215 2335118..2335525 - 408 Protein_1927 DDE-type integrase/transposase/recombinase -
AQ15_RS09615 2336171..2336455 - 285 WP_004525420.1 BrnA antitoxin family protein -
AQ15_RS09620 2336439..2336612 - 174 WP_009939402.1 BrnT family toxin -
AQ15_RS09625 2337280..2337555 + 276 WP_004552388.1 bacteriophage protein Gp49 -
AQ15_RS09630 2337565..2337696 + 132 WP_009923452.1 bacteriophage protein Gp48 -
AQ15_RS09640 2337832..2338053 + 222 WP_004552390.1 hypothetical protein -
AQ15_RS40335 2338020..2338319 + 300 WP_160469016.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2313777..2343096 29319
flank IS/Tn - - 2335118..2335438 320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-126)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6618.82 Da        Isoelectric Point: 11.2336

>T49223 WP_009893919.1 NZ_CP009537:2332934-2333113 [Burkholderia pseudomallei K96243]
MNSSKLIRMLEEDGWRLVRVTGSHHHFKHPKKPGLVTVPHPKKDLPIGTVKSIQKSAGL

Download         Length: 180 bp

>T49223 NZ_CP009537:2332934-2333113 [Burkholderia pseudomallei K96243]
ATGAACTCATCGAAGCTGATCCGGATGCTTGAGGAAGATGGCTGGAGGTTGGTTCGAGTGACAGGCAGCCATCATCATTT
CAAACACCCCAAGAAGCCAGGCCTTGTGACGGTTCCCCACCCGAAGAAGGACCTACCGATTGGGACTGTAAAAAGCATCC
AGAAATCCGCCGGCCTGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15120.06 Da        Isoelectric Point: 4.7025

>AT49223 WP_004552310.1 NZ_CP009537:2333158-2333574 [Burkholderia pseudomallei K96243]
MEFPIAVHKDDGSVYGVTVPDIPGVHSWGETIDDAIKNTREAIVGHVETLIELGEDVEFTCSTVEELVAKPEYAGAVWAL
VSVDLSQLDSKPERINVSIPRFVLHKIDAYVASRHETRSGFLARAALEALNEGKVRHA

Download         Length: 417 bp

>AT49223 NZ_CP009537:2333158-2333574 [Burkholderia pseudomallei K96243]
ATGGAATTTCCCATCGCAGTGCATAAGGACGATGGGAGCGTCTACGGCGTGACGGTCCCGGATATTCCGGGCGTTCACTC
CTGGGGCGAAACCATCGATGATGCAATCAAAAACACGAGAGAGGCTATCGTCGGCCATGTAGAGACATTGATCGAGCTTG
GAGAAGATGTTGAATTCACTTGCTCGACGGTGGAAGAACTGGTCGCAAAGCCCGAGTACGCCGGGGCGGTTTGGGCGCTC
GTCAGCGTTGATCTTTCTCAGCTTGATTCCAAGCCCGAGCGGATCAATGTGAGTATCCCTCGCTTCGTGCTGCACAAGAT
TGACGCCTATGTGGCATCCCGCCACGAGACTAGAAGCGGATTTCTTGCGCGTGCTGCCCTGGAGGCACTTAATGAAGGCA
AAGTTAGGCACGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4C26
PDB 6G26

Antitoxin

Source ID Structure
PDB 6G1C
PDB 6G1N
PDB 6G26
AlphaFold DB Q63NA5

References