Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) BrnTA/RHH-COG2929
Location 539214..539703 Replicon chromosome
Accession NC_006351
Organism Burkholderia pseudomallei K96243

Toxin (Protein)


Gene name brnT Uniprot ID -
Locus tag BPSS0395 Protein ID WP_009939402.1
Coordinates 539482..539655 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag BPSS0394 Protein ID WP_004525420.1
Coordinates 539214..539498 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BPS_RS20805 (BPSS0388) 534439..535173 - 735 WP_004552313.1 VRR-NUC domain-containing protein -
BPS_RS20810 (BPSS0389) 535170..535697 - 528 WP_004552312.1 PAAR domain-containing protein -
BPS_RS20815 (BPSS0390) 535977..536156 + 180 WP_009893919.1 type II toxin-antitoxin system HicA family toxin -
BPS_RS20820 (BPSS0391) 536201..536617 + 417 WP_004552310.1 type II toxin-antitoxin system HicB family antitoxin -
BPS_RS20825 (BPSS0391A) 537046..537543 - 498 WP_004552308.1 hypothetical protein -
BPS_RS20830 (BPSS0392) 538161..538568 - 408 Protein_443 DDE-type integrase/transposase/recombinase -
BPS_RS20835 (BPSS0394) 539214..539498 - 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
BPS_RS20840 (BPSS0395) 539482..539655 - 174 WP_009939402.1 BrnT family toxin Toxin
BPS_RS20845 (BPSS0396) 540323..540598 + 276 WP_004552388.1 bacteriophage protein Gp49 -
BPS_RS20850 (BPSS0397) 540608..540739 + 132 WP_009923452.1 bacteriophage protein Gp48 -
BPS_RS20855 (BPSS0398) 540875..541096 + 222 WP_004552390.1 hypothetical protein -
BPS_RS20860 541063..541362 + 300 WP_160469016.1 hypothetical protein -
BPS_RS20865 (BPSS0400) 541537..542127 - 591 WP_038717720.1 hypothetical protein -
BPS_RS20870 (BPSS0401) 542377..542526 + 150 WP_004537646.1 bacteriophage protein Gp44 -
BPS_RS20875 (BPSS0402) 542523..543296 + 774 Protein_452 prohibitin family protein -
BPS_RS32135 543294..543698 + 405 Protein_453 DDE-type integrase/transposase/recombinase -
BPS_RS20880 (BPSS0402A) 543661..544089 + 429 Protein_454 IS110 family transposase -
BPS_RS20885 (BPSS0402B) 544316..544432 - 117 Protein_455 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 516820..546139 29319
flank IS/Tn - - 538161..538481 320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(4-49)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6691.59 Da        Isoelectric Point: 7.9950

>T1547 WP_009939402.1 NC_006351:c539655-539482 [Burkholderia pseudomallei K96243]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Download         Length: 174 bp

>T1547 NC_006351:c539655-539482 [Burkholderia pseudomallei K96243]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT1547 WP_004525420.1 NC_006351:c539498-539214 [Burkholderia pseudomallei K96243]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT1547 NC_006351:c539498-539214 [Burkholderia pseudomallei K96243]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGTTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value
AT2224 Brucella melitensis biovar Abortus 2308

44.444

100

0.468
AT6121 Brucella melitensis biovar Abortus 2308

44.444

100

0.468
AT10188 Acinetobacter baumannii strain ORAB01

33.696

97.872

0.33
AT6081 Acinetobacter baumannii

33.696

97.872

0.33
AT6082 Acinetobacter baumannii

33.696

97.872

0.33

Multiple sequence alignment    




Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References

(1) Brittany N Ross et al. (2019) Evaluating the role of Burkholderia pseudomallei K96243 toxins BPSS0390, BPSS0395, and BPSS1584 in persistent infection. Cellular Microbiology 21(12):e13096. [PubMed:31400259] experimental


experimental experimental literature