196865

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 2281182..2281671 Replicon chromosome
Accession NZ_CP071760
Organism Burkholderia pseudomallei strain VBP21885

Toxin (Protein)


Gene name brnT Uniprot ID Q63NA2
Locus tag J3J51_RS27435 Protein ID WP_004547086.1
Coordinates 2281450..2281671 (-) Length 74 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag J3J51_RS27430 Protein ID WP_004525420.1
Coordinates 2281182..2281466 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J3J51_RS27410 (J3J51_27410) 2277503..2277823 + 321 WP_004196029.1 DUF4212 domain-containing protein -
J3J51_RS27415 (J3J51_27415) 2277817..2279832 + 2016 WP_004554778.1 VC_2705 family sodium/solute symporter -
J3J51_RS27425 (J3J51_27425) 2280128..2280535 - 408 Protein_1887 integrase core domain-containing protein -
J3J51_RS27430 (J3J51_27430) 2281182..2281466 - 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
J3J51_RS27435 (J3J51_27435) 2281450..2281671 - 222 WP_004547086.1 BrnT family toxin Toxin
J3J51_RS27440 (J3J51_27440) 2282291..2282566 + 276 WP_025988352.1 hypothetical protein -
J3J51_RS27445 (J3J51_27445) 2282576..2282707 + 132 WP_009923452.1 bacteriophage protein Gp48 -
J3J51_RS27450 (J3J51_27450) 2282843..2283064 + 222 WP_004525418.1 hypothetical protein -
J3J51_RS27455 (J3J51_27455) 2283031..2283330 + 300 WP_160973881.1 hypothetical protein -
J3J51_RS27460 (J3J51_27460) 2283505..2284095 - 591 WP_009946442.1 hypothetical protein -
J3J51_RS27465 (J3J51_27465) 2284345..2284494 + 150 WP_004537646.1 bacteriophage protein Gp44 -
J3J51_RS27470 (J3J51_27470) 2284530..2285063 + 534 Protein_1896 SPFH domain-containing protein -
J3J51_RS27475 (J3J51_27475) 2285262..2285666 + 405 Protein_1897 integrase core domain-containing protein -
J3J51_RS27480 (J3J51_27480) 2285665..2286057 + 393 Protein_1898 IS110 family transposase -
J3J51_RS27485 (J3J51_27485) 2286284..2286400 - 117 Protein_1899 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2277817..2285666 7849
flank IS/Tn - - 2280128..2280448 320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(14-65)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 74 a.a.        Molecular weight: 8634.77 Da        Isoelectric Point: 8.4920

>T196865 WP_004547086.1 NZ_CP071760:c2281671-2281450 [Burkholderia pseudomallei]
MECRWRLQRNSTGQDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Download         Length: 222 bp

>T196865 NZ_CP095497:c1474032-1473667 [Burkholderia cepacia]
ATGGTAAAAGCTCTCTTCGATACCAACATCCTGATCGACTATCTGGGCGGCGTGGAATCCGCTCGTGCGGAACTGGGCCG
CTACGACTATCGGGCGATCAGCACGATCTCGTGGATGGAGGTCCTGGCCGGCACGTCCGCACAGGACGAGGCACCCATCC
GCGCGTGGCTGTCGTCGTTCGATGTCATCGCACTGGACAGCCCGGTTGCGAATCGTGCCGTGGCAATCCGCAAGGAACGC
CGCATACGCCTTCCCGATGCAATCGTCTGGGCATCGGCACAAGTGAACGGTCTACTGCTCGTGTCGCGCAACACGAAGGA
CTTCCCTGCCACCGAACCCGGCGTTCGCGTGCCCTACAAGCTCTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT196865 WP_004525420.1 NZ_CP071760:c2281466-2281182 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT196865 NZ_CP095497:c1474244-1474026 [Burkholderia cepacia]
ATGAGCCGAATTCTGGTCGATCTGTCGGACGGCCAACTCGACGAGTTGGCCGTCATCGTCGAGACTCAACATCGTCCCCG
CGCCGCAATCATCCGCGACGCGATCGATGCCTATATTGCCTTGCACAAGCACCGGCTTGCCGACGACGTCTTCGGTCTCT
GGAAAGATCGCAAGGTCGACGGGCTCACCTATCAGGAAGAACTGCGCTCGGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q63NA2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References