Detailed information of TA system    

experimentalExperimentally validated    

Overview


TA module


Type II Classification (family/domain) BrnTA/RHH-COG2929
Location 959245..959879 Replicon chromosome
Accession NC_007618
Organism Brucella melitensis biovar Abortus 2308

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag BAB_RS20710 Protein ID WP_002966807.1
Coordinates 959559..959804 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q57DF1
Locus tag BAB_RS20705 Protein ID WP_002964097.1
Coordinates 959245..959559 (-) Length 105 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAB_RS20650 954937..955140 - 204 WP_002964087.1 hypothetical protein -
BAB_RS20655 955143..955358 - 216 WP_002971459.1 hypothetical protein -
BAB_RS20660 955355..955558 - 204 WP_002964088.1 hypothetical protein -
BAB_RS32510 955608..956326 + 719 Protein_918 hypothetical protein -
BAB_RS20675 (BAB1_0986) 956323..956553 + 231 WP_002964091.1 hypothetical protein -
BAB_RS20680 (BAB1_0987) 956550..956825 + 276 WP_002964092.1 hypothetical protein -
BAB_RS20685 (BAB1_0988) 956848..957324 + 477 WP_002966806.1 hypothetical protein -
BAB_RS32515 957670..958379 + 710 Protein_922 porin family protein -
BAB_RS20700 (BAB1_0991) 958727..958897 - 171 WP_002964095.1 DUF1508 domain-containing protein -
BAB_RS20705 (BAB1_0993) 959245..959559 - 315 WP_002964097.1 BrnA antitoxin family protein Antitoxin
BAB_RS20710 (BAB1_0994) 959559..959804 - 246 WP_002966807.1 BrnT family toxin Toxin
BAB_RS20715 (BAB1_0995) 959937..960575 + 639 Protein_926 tRNA-guanine transglycosylase -
BAB_RS33640 960645..961402 + 758 WP_102040076.1 IS5 family transposase -
BAB_RS33645 961403..962176 + 774 Protein_928 hypothetical protein -
BAB_RS20740 (BAB1_0998) 962505..963956 + 1452 WP_004685623.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - wboA / wbdA 953925..967272 13347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T6121 WP_002966807.1 NC_007618:c959804-959559 [Brucella abortus 2308]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T6121 NC_007618:c959804-959559 [Brucella abortus 2308]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCTTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11638.42 Da        Isoelectric Point: 10.3131

>AT6121 WP_002964097.1 NC_007618:c959559-959245 [Brucella abortus 2308]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT6121 NC_007618:c959559-959245 [Brucella abortus 2308]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T2224 Brucella melitensis biovar Abortus 2308

100

100

1

T6082 Acinetobacter baumannii

31.325

100

0.321

T10188 Acinetobacter baumannii strain ORAB01

31.325

100

0.321

T6081 Acinetobacter baumannii

31.325

100

0.321

T6086 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

28.736

100

0.309


Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT2224 Brucella melitensis biovar Abortus 2308

100

100

1

AT1547 Burkholderia pseudomallei K96243

44.444

100

0.468

AT6082 Acinetobacter baumannii

30.357

100

0.333

AT6081 Acinetobacter baumannii

30.357

100

0.333

AT10188 Acinetobacter baumannii strain ORAB01

30.357

100

0.327


Multiple sequence alignment    




Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14


Antitoxin

Source ID Structure

References


(1) Brook E Heaton et al. (2012) Molecular structure and function of the novel BrnT/BrnA toxin-antitoxin system of Brucella abortus. The Journal of Biological Chemistry 287(15):12098-110. [PubMed:22334680] experimental


experimental experimental literature