113071

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 872983..873542 Replicon chromosome
Accession NZ_CP033079
Organism Brucella abortus strain BJ1

Toxin (Protein)


Gene name brnT Uniprot ID D7H3C9
Locus tag EAI02_RS04625 Protein ID WP_002964098.1
Coordinates 873297..873542 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q57DF1
Locus tag EAI02_RS04620 Protein ID WP_002964097.1
Coordinates 872983..873297 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAI02_RS04570 868675..868878 - 204 WP_002964087.1 hypothetical protein -
EAI02_RS04575 868881..869096 - 216 WP_002971459.1 hypothetical protein -
EAI02_RS04580 869093..869296 - 204 WP_002964088.1 hypothetical protein -
EAI02_RS04585 869346..870064 + 719 Protein_843 hypothetical protein -
EAI02_RS04590 870061..870291 + 231 WP_002964091.1 hypothetical protein -
EAI02_RS04595 870288..870563 + 276 WP_002964092.1 hypothetical protein -
EAI02_RS04600 870586..871062 + 477 WP_002966806.1 hypothetical protein -
EAI02_RS04605 871408..872117 + 710 Protein_847 porin family protein -
EAI02_RS04610 872465..872635 - 171 WP_002964095.1 DUF1508 domain-containing protein -
EAI02_RS04620 872983..873297 - 315 WP_002964097.1 BrnA antitoxin family protein Antitoxin
EAI02_RS04625 873297..873542 - 246 WP_002964098.1 BrnT family toxin Toxin
EAI02_RS04630 873675..874313 + 639 Protein_851 tRNA-guanine transglycosylase -
EAI02_RS04635 874383..875140 + 758 WP_094866390.1 IS5 family transposase -
EAI02_RS04640 875141..875914 + 774 WP_002967634.1 hypothetical protein -
EAI02_RS04645 876243..877694 + 1452 WP_004685623.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - wboA / wbdA 866903..881010 14107


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9196.83 Da        Isoelectric Point: 10.3158

>T113071 WP_002964098.1 NZ_CP033079:c873542-873297 [Brucella abortus]
MKIFWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T113071 NZ_CP033079:c873542-873297 [Brucella abortus]
ATGAAGATCTTCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCTTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11638.42 Da        Isoelectric Point: 10.3131

>AT113071 WP_002964097.1 NZ_CP033079:c873297-872983 [Brucella abortus]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT113071 NZ_CP033079:c873297-872983 [Brucella abortus]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D7H3C9

Antitoxin

Source ID Structure

References