144748

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 959241..959800 Replicon chromosome
Accession NZ_CP046720
Organism Brucella abortus RB51-AHVLA strain RB51

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag F6460_RS04750 Protein ID WP_002966807.1
Coordinates 959555..959800 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q57DF1
Locus tag F6460_RS04745 Protein ID WP_002964097.1
Coordinates 959241..959555 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6460_RS04700 954933..955136 - 204 WP_002964087.1 hypothetical protein -
F6460_RS04705 955139..955354 - 216 WP_002971459.1 hypothetical protein -
F6460_RS04710 955351..955554 - 204 WP_002964088.1 hypothetical protein -
F6460_RS04715 955604..956322 + 719 Protein_918 hypothetical protein -
F6460_RS04720 956319..956549 + 231 WP_002964091.1 hypothetical protein -
F6460_RS04725 956546..956821 + 276 WP_002964092.1 hypothetical protein -
F6460_RS04730 956844..957320 + 477 WP_002966806.1 hypothetical protein -
F6460_RS04735 957666..958375 + 710 Protein_922 porin family protein -
F6460_RS04740 958723..958893 - 171 WP_002964095.1 DUF1508 domain-containing protein -
F6460_RS04745 959241..959555 - 315 WP_002964097.1 BrnA antitoxin family protein Antitoxin
F6460_RS04750 959555..959800 - 246 WP_002966807.1 BrnT family toxin Toxin
F6460_RS04755 959933..960571 + 639 Protein_926 tRNA-guanine transglycosylase -
F6460_RS04760 960641..961398 + 758 WP_102040076.1 IS5 family transposase -
F6460_RS04765 961399..962172 + 774 Protein_928 hypothetical protein -
F6460_RS04770 962501..963952 + 1452 WP_004685623.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - wboA / wbdA 953921..968112 14191


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T144748 WP_002966807.1 NZ_CP046720:c959800-959555 [Brucella abortus RB51-AHVLA]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T144748 NZ_CP062239:4438509-4438859 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11638.42 Da        Isoelectric Point: 10.3131

>AT144748 WP_002964097.1 NZ_CP046720:c959555-959241 [Brucella abortus RB51-AHVLA]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT144748 NZ_CP062239:4438264-4438515 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure

References