49338

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 961708..962267 Replicon chromosome
Accession NZ_CP009625
Organism Brucella abortus 104M

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag NL70_RS04745 Protein ID WP_002966807.1
Coordinates 962022..962267 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q57DF1
Locus tag NL70_RS04740 Protein ID WP_002964097.1
Coordinates 961708..962022 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL70_RS04685 957400..957603 - 204 WP_002964087.1 hypothetical protein -
NL70_RS04690 957606..957821 - 216 WP_002971459.1 hypothetical protein -
NL70_RS04695 957818..958021 - 204 WP_002964088.1 hypothetical protein -
NL70_RS04700 958071..958771 + 701 Protein_918 hypothetical protein -
NL70_RS04705 958786..959016 + 231 WP_002964091.1 hypothetical protein -
NL70_RS04710 959013..959288 + 276 WP_002964092.1 hypothetical protein -
NL70_RS04715 959311..959787 + 477 WP_002966806.1 hypothetical protein -
NL70_RS16480 960133..960842 + 710 Protein_922 porin family protein -
NL70_RS04730 961190..961360 - 171 WP_002964095.1 DUF1508 domain-containing protein -
NL70_RS04740 961708..962022 - 315 WP_002964097.1 BrnA antitoxin family protein Antitoxin
NL70_RS04745 962022..962267 - 246 WP_002966807.1 BrnT family toxin Toxin
NL70_RS04750 962400..963038 + 639 Protein_926 tRNA-guanine transglycosylase -
NL70_RS17615 963108..963865 + 758 WP_102040076.1 IS5 family transposase -
NL70_RS17620 963866..964639 + 774 Protein_928 phage portal protein -
NL70_RS04775 964968..966419 + 1452 WP_004685623.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - wboA / wbdA 955628..969735 14107


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T49338 WP_002966807.1 NZ_CP009625:c962267-962022 [Brucella abortus 104M]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T49338 NZ_CP009625:c962267-962022 [Brucella abortus 104M]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCTTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11638.42 Da        Isoelectric Point: 10.3131

>AT49338 WP_002964097.1 NZ_CP009625:c962022-961708 [Brucella abortus 104M]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT49338 NZ_CP009625:c962022-961708 [Brucella abortus 104M]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure

References