46968

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 1075106..1075665 Replicon chromosome
Accession NZ_CP007738
Organism Brucella abortus strain BFY

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag DO75_RS10615 Protein ID WP_002966807.1
Coordinates 1075106..1075351 (+) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q57DF1
Locus tag DO75_RS10620 Protein ID WP_002964097.1
Coordinates 1075351..1075665 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DO75_RS10590 1070954..1072405 - 1452 WP_004685623.1 hypothetical protein -
DO75_RS10595 1072734..1073507 - 774 WP_002967634.1 hypothetical protein -
DO75_RS10610 1074335..1074973 - 639 Protein_1014 tRNA-guanine transglycosylase -
DO75_RS10615 1075106..1075351 + 246 WP_002966807.1 BrnT family toxin Toxin
DO75_RS10620 1075351..1075665 + 315 WP_002964097.1 BrnA antitoxin family protein Antitoxin
DO75_RS10625 1076013..1076183 + 171 WP_002964095.1 DUF1508 domain-containing protein -
DO75_RS16790 1076531..1077240 - 710 Protein_1018 porin family protein -
DO75_RS10640 1077586..1078062 - 477 WP_002966806.1 hypothetical protein -
DO75_RS10645 1078085..1078359 - 275 Protein_1020 hypothetical protein -
DO75_RS10650 1078356..1078586 - 231 WP_002964091.1 hypothetical protein -
DO75_RS10655 1078583..1079302 - 720 WP_006103139.1 hypothetical protein -
DO75_RS10660 1079352..1079555 + 204 WP_002964088.1 hypothetical protein -
DO75_RS10665 1079552..1079767 + 216 WP_002971459.1 hypothetical protein -
DO75_RS10670 1079770..1079973 + 204 WP_002964087.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - wbdA / wboA 1067840..1081745 13905


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T46968 WP_002966807.1 NZ_CP007738:1075106-1075351 [Brucella abortus]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T46968 NZ_CP007738:1075106-1075351 [Brucella abortus]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCTTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11638.42 Da        Isoelectric Point: 10.3131

>AT46968 WP_002964097.1 NZ_CP007738:1075351-1075665 [Brucella abortus]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT46968 NZ_CP007738:1075351-1075665 [Brucella abortus]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure

References