46886

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 1165571..1166130 Replicon chromosome
Accession NZ_CP007681
Organism Brucella abortus strain BDW

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag DK48_RS11175 Protein ID WP_002966807.1
Coordinates 1165571..1165816 (+) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q57DF1
Locus tag DK48_RS11180 Protein ID WP_002964097.1
Coordinates 1165816..1166130 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DK48_RS11145 1161419..1162870 - 1452 WP_004685623.1 hypothetical protein -
DK48_RS17910 1163199..1163972 - 774 Protein_1098 hypothetical protein -
DK48_RS11170 1164800..1165438 - 639 Protein_1100 tRNA-guanine transglycosylase -
DK48_RS11175 1165571..1165816 + 246 WP_002966807.1 BrnT family toxin Toxin
DK48_RS11180 1165816..1166130 + 315 WP_002964097.1 BrnA antitoxin family protein Antitoxin
DK48_RS11185 1166478..1166648 + 171 WP_002964095.1 DUF1508 domain-containing protein -
DK48_RS17045 1166996..1167705 - 710 Protein_1104 porin family protein -
DK48_RS11200 1168051..1168527 - 477 WP_002966806.1 hypothetical protein -
DK48_RS11205 1168550..1168825 - 276 WP_002964092.1 hypothetical protein -
DK48_RS11210 1168822..1169052 - 231 WP_002964091.1 hypothetical protein -
DK48_RS17050 1169049..1169767 - 719 Protein_1108 hypothetical protein -
DK48_RS11225 1169817..1170020 + 204 WP_002964088.1 hypothetical protein -
DK48_RS11230 1170017..1170232 + 216 WP_002971459.1 hypothetical protein -
DK48_RS11235 1170235..1170438 + 204 WP_002964087.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - wbdA / wboA 1158305..1172210 13905


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T46886 WP_002966807.1 NZ_CP007681:1165571-1165816 [Brucella abortus]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T46886 NZ_CP007681:1165571-1165816 [Brucella abortus]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCTTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11638.42 Da        Isoelectric Point: 10.3131

>AT46886 WP_002964097.1 NZ_CP007681:1165816-1166130 [Brucella abortus]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT46886 NZ_CP007681:1165816-1166130 [Brucella abortus]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure

References