47421

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 953225..953784 Replicon chromosome
Accession NZ_CP008774
Organism Brucella abortus strain BAB8416

Toxin (Protein)


Gene name brnT Uniprot ID D7H3C9
Locus tag BAB8416_RS04705 Protein ID WP_002964098.1
Coordinates 953539..953784 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q57DF1
Locus tag BAB8416_RS04700 Protein ID WP_002964097.1
Coordinates 953225..953539 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAB8416_RS04645 948917..949120 - 204 WP_002964087.1 hypothetical protein -
BAB8416_RS04650 949123..949338 - 216 WP_002971459.1 hypothetical protein -
BAB8416_RS04655 949335..949538 - 204 WP_002964088.1 hypothetical protein -
BAB8416_RS16285 949588..950306 + 719 Protein_910 hypothetical protein -
BAB8416_RS04670 950303..950533 + 231 WP_002964091.1 hypothetical protein -
BAB8416_RS04675 950530..950805 + 276 WP_002964092.1 hypothetical protein -
BAB8416_RS04680 950828..951304 + 477 WP_002966806.1 hypothetical protein -
BAB8416_RS16290 951650..952359 + 710 Protein_914 porin family protein -
BAB8416_RS04695 952707..952877 - 171 WP_002964095.1 DUF1508 domain-containing protein -
BAB8416_RS04700 953225..953539 - 315 WP_002964097.1 BrnA antitoxin family protein Antitoxin
BAB8416_RS04705 953539..953784 - 246 WP_002964098.1 BrnT family toxin Toxin
BAB8416_RS04710 953917..954555 + 639 Protein_918 tRNA-guanine transglycosylase -
BAB8416_RS17420 954625..955383 + 759 Protein_919 IS5 family transposase -
BAB8416_RS04720 955384..956157 + 774 WP_002967634.1 hypothetical protein -
BAB8416_RS04725 956486..957937 + 1452 WP_004685623.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - wboA / wbdA 947145..961253 14108
- flank IS/Tn - - 954625..954993 368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9196.83 Da        Isoelectric Point: 10.3158

>T47421 WP_002964098.1 NZ_CP008774:c953784-953539 [Brucella abortus]
MKIFWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T47421 NZ_CP008774:c953784-953539 [Brucella abortus]
ATGAAGATCTTCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCTTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11638.42 Da        Isoelectric Point: 10.3131

>AT47421 WP_002964097.1 NZ_CP008774:c953539-953225 [Brucella abortus]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT47421 NZ_CP008774:c953539-953225 [Brucella abortus]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D7H3C9

Antitoxin

Source ID Structure

References