107834

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 961892..962451 Replicon chromosome
Accession NZ_CP030751
Organism Brucella abortus strain A19

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag DR186_RS05055 Protein ID WP_002966807.1
Coordinates 962206..962451 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q57DF1
Locus tag DR186_RS05050 Protein ID WP_002964097.1
Coordinates 961892..962206 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DR186_RS05000 957584..957787 - 204 WP_002964087.1 hypothetical protein -
DR186_RS05005 957790..958005 - 216 WP_002971459.1 hypothetical protein -
DR186_RS05010 958002..958205 - 204 WP_002964088.1 hypothetical protein -
DR186_RS05015 958255..958973 + 719 Protein_917 hypothetical protein -
DR186_RS05020 958970..959200 + 231 WP_002964091.1 hypothetical protein -
DR186_RS05025 959197..959472 + 276 WP_002964092.1 hypothetical protein -
DR186_RS05030 959495..959971 + 477 WP_002966806.1 hypothetical protein -
DR186_RS05035 960317..961026 + 710 Protein_921 porin family protein -
DR186_RS05040 961374..961544 - 171 WP_002964095.1 DUF1508 domain-containing protein -
DR186_RS05050 961892..962206 - 315 WP_002964097.1 BrnA antitoxin family protein Antitoxin
DR186_RS05055 962206..962451 - 246 WP_002966807.1 BrnT family toxin Toxin
DR186_RS05060 962584..963222 + 639 Protein_925 tRNA-guanine transglycosylase -
DR186_RS05065 963292..964049 + 758 WP_102040076.1 IS5 family transposase -
DR186_RS05070 964050..964823 + 774 Protein_927 hypothetical protein -
DR186_RS05075 965152..966603 + 1452 WP_004685623.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - wboA / wbdA 956572..969919 13347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T107834 WP_002966807.1 NZ_CP030751:c962451-962206 [Brucella abortus]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T107834 NZ_CP030751:c962451-962206 [Brucella abortus]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCTTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11638.42 Da        Isoelectric Point: 10.3131

>AT107834 WP_002964097.1 NZ_CP030751:c962206-961892 [Brucella abortus]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT107834 NZ_CP030751:c962206-961892 [Brucella abortus]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure

References