Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | AbkAB/BrnT(toxin) |
| Location | 3527..4124 | Replicon | plasmid AbATCC329 |
| Accession | KJ534569 | ||
| Organism | Acinetobacter baumannii | ||
Toxin (Protein)
| Gene name | AbkB | Uniprot ID | - |
| Locus tag | Locus_2 | Protein ID | AIA61630.1 |
| Coordinates | 3828..4124 (-) | Length | 99 a.a. |
Antitoxin (Protein)
| Gene name | AbkA | Uniprot ID | - |
| Locus tag | Locus_1 | Protein ID | AIA61629.1 |
| Coordinates | 3527..3835 (-) | Length | 103 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_0 | 987..3398 | - | 2412 | AIA61628.1 | TonB-dependent receptor | - |
| Locus_1 | 3527..3835 | - | 309 | AIA61629.1 | antitoxin component | Antitoxin |
| Locus_2 | 3828..4124 | - | 297 | AIA61630.1 | toxin component | Toxin |
| Locus_3 | 4366..5193 | + | 828 | AIA61631.1 | OXA24 B-lactamase | - |
| Locus_4 | 5376..5519 | - | 144 | AIA61632.1 | hypothetical protein | - |
| Locus_5 | 5531..6106 | - | 576 | AIA61633.1 | DNA replication protein | - |
| Locus_6 | 6099..7049 | - | 951 | AIA61634.1 | DNA replication protein | - |
| Locus_7 | 7654..7926 | + | 273 | AIA61635.1 | hypothetical protein | - |
| Locus_8 | 8197..8568 | + | 372 | AIA61636.1 | hypothetical protein | - |
| Locus_9 | 8568..8741 | + | 174 | AIA61637.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Non-Mobilizable plasmid | blaOXA-24 | - | 1..8842 | 8842 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 99 a.a. Molecular weight: 11729.26 Da Isoelectric Point: 6.0889
>T6082 AIA61630.1 KJ534569:c4124-3828 [Acinetobacter baumannii]
MFFMEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIR
IISARQVTKAERNKYEHG
MFFMEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIR
IISARQVTKAERNKYEHG
Download Length: 297 bp
>T6082 KJ534569:c4124-3828 [Acinetobacter baumannii]
ATGTTTTTTATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGA
AACTGCAAGCCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAA
CCATTGGACGAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGA
ATCATTAGTGCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG
ATGTTTTTTATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGA
AACTGCAAGCCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAA
CCATTGGACGAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGA
ATCATTAGTGCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG
Antitoxin
Download Length: 103 a.a. Molecular weight: 12009.61 Da Isoelectric Point: 8.4628
>AT6082 AIA61629.1 KJ534569:c3835-3527 [Acinetobacter baumannii]
MVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGK
GYQTRMNEILRDAMLKELKNHQ
MVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGK
GYQTRMNEILRDAMLKELKNHQ
Download Length: 309 bp
>AT6082 KJ534569:c3835-3527 [Acinetobacter baumannii]
ATGGTTAGATACTCACGCAAAGAGCTAAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCTTGCTAA
AGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCCAAAATC
AATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAAGGCAAA
GGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA
ATGGTTAGATACTCACGCAAAGAGCTAAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCTTGCTAA
AGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCCAAAATC
AATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAAGGCAAA
GGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6081 | Acinetobacter baumannii |
100 |
100 |
1 |
| T10188 | Acinetobacter baumannii strain ORAB01 |
100 |
100 |
1 |
| T6086 | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
52.632 |
100 |
0.526 |
| T167 | Vibrio cholerae O1 biovar El Tor str. N16961 |
37.079 |
100 |
0.375 |
| T2224 | Brucella melitensis biovar Abortus 2308 |
31.325 |
100 |
0.321 |
| T6121 | Brucella melitensis biovar Abortus 2308 |
31.325 |
100 |
0.321 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6081 | Acinetobacter baumannii |
100 |
100 |
1 |
| AT10188 | Acinetobacter baumannii strain ORAB01 |
99.02 |
100 |
0.99 |
| AT6086 | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
48.485 |
99 |
0.48 |
| AT6121 | Brucella melitensis biovar Abortus 2308 |
30.357 |
100 |
0.333 |
| AT2224 | Brucella melitensis biovar Abortus 2308 |
30.357 |
100 |
0.333 |
| AT1547 | Burkholderia pseudomallei K96243 |
33.696 |
97.872 |
0.33 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Noraida Mosqueda et al. (2014) Characterization of plasmids carrying the blaOXA-24/40 carbapenemase gene and the genes encoding the AbkA/AbkB proteins of a toxin/antitoxin system. The Journal of Antimicrobial Chemotherapy 69(10):2629-33. [PubMed:24879663]
(2) Alireza Japoni-Nejad et al. (2021) Identification and characterization of the type II toxin-antitoxin systems in the carbapenem-resistant Acinetobacterbaumannii. Microbial Pathogenesis 158:105052. [PubMed:34147586]
(3) Laura Fernández-García et al. (2018) Relationship between Tolerance and Persistence Mechanisms in Acinetobacter baumannii Strains with AbkAB Toxin-Antitoxin System. Antimicrobial Agents And Chemotherapy 62(5):e00250-18. [PubMed:29463538]