Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | BrnTA/- |
| Location | 11267..11855 | Replicon | plasmid pORAB01-3 |
| Accession | NZ_CP015486 | ||
| Organism | Acinetobacter baumannii strain ORAB01 | ||
Toxin (Protein)
| Gene name | brnT | Uniprot ID | A0A3G6Z3M6 |
| Locus tag | SG90_RS19795 | Protein ID | WP_000438826.1 |
| Coordinates | 11267..11554 (+) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | brnA | Uniprot ID | N9M5I3 |
| Locus tag | SG90_RS19800 | Protein ID | WP_001983304.1 |
| Coordinates | 11541..11855 (+) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SG90_RS19780 (SG90_019780) | 8318..9268 | + | 951 | WP_001205341.1 | replication initiation protein RepM | - |
| SG90_RS19785 (SG90_019785) | 9261..9836 | + | 576 | WP_001096611.1 | helix-turn-helix domain-containing protein | - |
| SG90_RS20740 | 9858..10001 | + | 144 | WP_000734097.1 | hypothetical protein | - |
| SG90_RS19790 (SG90_019790) | 10184..10957 | - | 774 | WP_001022900.1 | alpha/beta hydrolase | - |
| SG90_RS19795 (SG90_019795) | 11267..11554 | + | 288 | WP_000438826.1 | BrnT family toxin | Toxin |
| SG90_RS19800 (SG90_019800) | 11541..11855 | + | 315 | WP_001983304.1 | BrnA antitoxin family protein | Antitoxin |
| SG90_RS19815 (SG90_019815) | 13126..13956 | + | 831 | WP_000854010.1 | OXA-134 family carbapenem-hydrolyzing class D beta-lactamase OXA-237 | - |
| SG90_RS20470 | 14090..15180 | + | 1091 | WP_085940413.1 | IS4-like element ISAba1 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | blaOXA-237 | - | 1..15198 | 15198 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11303.72 Da Isoelectric Point: 6.0889
>T10188 WP_000438826.1 NZ_CP015486:11267-11554 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
Download Length: 288 bp
>T10188 NZ_CP015486:11267-11554 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG
Antitoxin
Download Length: 105 a.a. Molecular weight: 12208.84 Da Isoelectric Point: 7.2026
>AT10188 WP_001983304.1 NZ_CP015486:11541-11855 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
Download Length: 315 bp
>AT10188 NZ_CP015486:11541-11855 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6081 | Acinetobacter baumannii |
100 |
100 |
1 |
| T6082 | Acinetobacter baumannii |
100 |
100 |
1 |
| T6086 | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
52.632 |
100 |
0.526 |
| T167 | Vibrio cholerae O1 biovar El Tor str. N16961 |
37.079 |
100 |
0.375 |
| T6121 | Brucella melitensis biovar Abortus 2308 |
31.325 |
100 |
0.321 |
| T2224 | Brucella melitensis biovar Abortus 2308 |
31.325 |
100 |
0.321 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6082 | Acinetobacter baumannii |
99.02 |
100 |
0.99 |
| AT6081 | Acinetobacter baumannii |
99.02 |
100 |
0.99 |
| AT6086 | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
50.98 |
100 |
0.52 |
| AT1547 | Burkholderia pseudomallei K96243 |
33.696 |
97.872 |
0.33 |
| AT2224 | Brucella melitensis biovar Abortus 2308 |
30.357 |
100 |
0.327 |
| AT6121 | Brucella melitensis biovar Abortus 2308 |
30.357 |
100 |
0.327 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3G6Z3M6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | N9M5I3 |
References
(1) Andrea M Hujer et al. (2017) Nosocomial Outbreak of Extensively Drug-Resistant Acinetobacter baumannii Isolates Containing blaOXA-237 Carried on a Plasmid. Antimicrobial Agents And Chemotherapy 61(11):e00797-17. [PubMed:28893775]