79824

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 1162457..1163016 Replicon chromosome
Accession NZ_CP022877
Organism Brucella abortus strain BD

Toxin (Protein)


Gene name brnT Uniprot ID D7H3C9
Locus tag CJP69_RS06000 Protein ID WP_002964098.1
Coordinates 1162457..1162702 (+) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q57DF1
Locus tag CJP69_RS06005 Protein ID WP_002964097.1
Coordinates 1162702..1163016 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJP69_RS05980 1158360..1159811 - 1452 WP_004685623.1 hypothetical protein -
CJP69_RS05985 1160140..1160913 - 774 WP_002967634.1 hypothetical protein -
CJP69_RS05990 1160914..1161616 - 703 Protein_1097 IS5 family transposase -
CJP69_RS05995 1161686..1162324 - 639 Protein_1098 tRNA-guanine transglycosylase -
CJP69_RS06000 1162457..1162702 + 246 WP_002964098.1 BrnT family toxin Toxin
CJP69_RS06005 1162702..1163016 + 315 WP_002964097.1 BrnA antitoxin family protein Antitoxin
CJP69_RS06015 1163364..1163534 + 171 WP_002964095.1 DUF1508 domain-containing protein -
CJP69_RS06020 1163882..1164591 - 710 Protein_1102 porin family protein -
CJP69_RS06025 1164937..1165413 - 477 WP_002966806.1 hypothetical protein -
CJP69_RS06030 1165436..1165711 - 276 WP_002964092.1 hypothetical protein -
CJP69_RS06035 1165708..1165938 - 231 WP_002964091.1 hypothetical protein -
CJP69_RS06040 1165935..1166653 - 719 Protein_1106 hypothetical protein -
CJP69_RS06045 1166703..1166906 + 204 WP_002964088.1 hypothetical protein -
CJP69_RS06050 1166903..1167118 + 216 WP_002971459.1 hypothetical protein -
CJP69_RS06055 1167121..1167324 + 204 WP_002964087.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - wbdA / wboA 1155246..1169096 13850
- flank IS/Tn - - 1161248..1161616 368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9196.83 Da        Isoelectric Point: 10.3158

>T79824 WP_002964098.1 NZ_CP022877:1162457-1162702 [Brucella abortus]
MKIFWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T79824 NZ_CP022877:1162457-1162702 [Brucella abortus]
ATGAAGATCTTCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCTTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11638.42 Da        Isoelectric Point: 10.3131

>AT79824 WP_002964097.1 NZ_CP022877:1162702-1163016 [Brucella abortus]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT79824 NZ_CP022877:1162702-1163016 [Brucella abortus]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D7H3C9

Antitoxin

Source ID Structure

References