46815

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 726771..727330 Replicon chromosome
Accession NZ_CP007663
Organism Brucella abortus strain 63 75

Toxin (Protein)


Gene name brnT Uniprot ID D7H3C9
Locus tag DK49_RS09150 Protein ID WP_002964098.1
Coordinates 727085..727330 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q57DF1
Locus tag DK49_RS09145 Protein ID WP_002964097.1
Coordinates 726771..727085 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DK49_RS09095 722464..722667 - 204 WP_002964087.1 hypothetical protein -
DK49_RS09100 722670..722885 - 216 WP_002971459.1 hypothetical protein -
DK49_RS09105 722882..723085 - 204 WP_002964088.1 hypothetical protein -
DK49_RS17985 723542..723852 + 311 Protein_714 hypothetical protein -
DK49_RS09115 723849..724079 + 231 WP_002964091.1 hypothetical protein -
DK49_RS09120 724076..724351 + 276 WP_002964092.1 hypothetical protein -
DK49_RS09125 724374..724850 + 477 WP_002966806.1 hypothetical protein -
DK49_RS16785 725196..725905 + 710 Protein_718 porin family protein -
DK49_RS09140 726253..726423 - 171 WP_002964095.1 DUF1508 domain-containing protein -
DK49_RS09145 726771..727085 - 315 WP_002964097.1 BrnA antitoxin family protein Antitoxin
DK49_RS09150 727085..727330 - 246 WP_002964098.1 BrnT family toxin Toxin
DK49_RS09155 727463..728101 + 639 Protein_722 tRNA-guanine transglycosylase -
DK49_RS17655 728171..728928 + 758 WP_094866390.1 IS5 family transposase -
DK49_RS09170 728929..729702 + 774 WP_002967634.1 hypothetical protein -
DK49_RS09175 730031..731482 + 1452 WP_032449309.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - wboA / wbdA 720692..734798 14106


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9196.83 Da        Isoelectric Point: 10.3158

>T46815 WP_002964098.1 NZ_CP007663:c727330-727085 [Brucella abortus]
MKIFWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

>T46815 NZ_CP007663:c727330-727085 [Brucella abortus]
ATGAAGATCTTCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCTTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11638.42 Da        Isoelectric Point: 10.3131

>AT46815 WP_002964097.1 NZ_CP007663:c727085-726771 [Brucella abortus]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp

>AT46815 NZ_CP007663:c727085-726771 [Brucella abortus]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D7H3C9

Antitoxin

Source ID Structure

References