196843

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 1453690..1454179 Replicon chromosome
Accession NZ_CP071750
Organism Burkholderia pseudomallei strain VBM21885

Toxin (Protein)


Gene name brnT Uniprot ID Q63NA2
Locus tag J3B47_RS24425 Protein ID WP_004547086.1
Coordinates 1453958..1454179 (-) Length 74 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag J3B47_RS24420 Protein ID WP_004525420.1
Coordinates 1453690..1453974 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J3B47_RS24400 (J3B47_24400) 1450011..1450331 + 321 WP_004196029.1 DUF4212 domain-containing protein -
J3B47_RS24405 (J3B47_24405) 1450325..1452340 + 2016 WP_004554778.1 VC_2705 family sodium/solute symporter -
J3B47_RS24415 (J3B47_24415) 1452636..1453043 - 408 Protein_1225 integrase core domain-containing protein -
J3B47_RS24420 (J3B47_24420) 1453690..1453974 - 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
J3B47_RS24425 (J3B47_24425) 1453958..1454179 - 222 WP_004547086.1 BrnT family toxin Toxin
J3B47_RS24430 (J3B47_24430) 1454799..1455074 + 276 WP_025988352.1 hypothetical protein -
J3B47_RS24435 (J3B47_24435) 1455084..1455215 + 132 WP_009923452.1 bacteriophage protein Gp48 -
J3B47_RS24440 (J3B47_24440) 1455351..1455572 + 222 WP_004525418.1 hypothetical protein -
J3B47_RS24445 (J3B47_24445) 1455539..1455838 + 300 WP_160973881.1 hypothetical protein -
J3B47_RS24450 (J3B47_24450) 1456013..1456603 - 591 WP_009946442.1 hypothetical protein -
J3B47_RS24455 (J3B47_24455) 1456853..1457002 + 150 WP_004537646.1 bacteriophage protein Gp44 -
J3B47_RS24460 (J3B47_24460) 1457038..1457571 + 534 Protein_1234 SPFH domain-containing protein -
J3B47_RS24465 (J3B47_24465) 1457770..1458174 + 405 Protein_1235 integrase core domain-containing protein -
J3B47_RS24470 (J3B47_24470) 1458173..1458565 + 393 Protein_1236 IS110 family transposase -
J3B47_RS24475 (J3B47_24475) 1458792..1458908 - 117 Protein_1237 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1449951..1458174 8223
flank IS/Tn - - 1452636..1452956 320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(14-65)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 74 a.a.        Molecular weight: 8634.77 Da        Isoelectric Point: 8.4920

>T196843 WP_004547086.1 NZ_CP071750:c1454179-1453958 [Burkholderia pseudomallei]
MECRWRLQRNSTGQDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Download         Length: 222 bp

>T196843 NZ_CP095448:4330299-4330556 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT196843 WP_004525420.1 NZ_CP071750:c1453974-1453690 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT196843 NZ_CP095448:4330568-4331113 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q63NA2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References