196871

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 2518891..2519380 Replicon chromosome
Accession NZ_CP071762
Organism Burkholderia pseudomallei strain VBP23831

Toxin (Protein)


Gene name brnT Uniprot ID Q63NA2
Locus tag J3L14_RS28395 Protein ID WP_004547086.1
Coordinates 2518891..2519112 (+) Length 74 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag J3L14_RS28400 Protein ID WP_004525420.1
Coordinates 2519096..2519380 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J3L14_RS28355 (J3L14_28355) 2514816..2515244 - 429 Protein_2081 integrase core domain-containing protein -
J3L14_RS28360 (J3L14_28360) 2515443..2515976 - 534 Protein_2082 SPFH domain-containing protein -
J3L14_RS28365 (J3L14_28365) 2516012..2516161 - 150 WP_004537646.1 bacteriophage protein Gp44 -
J3L14_RS28370 (J3L14_28370) 2516420..2517001 + 582 WP_004542548.1 hypothetical protein -
J3L14_RS28375 (J3L14_28375) 2517232..2517531 - 300 WP_195761357.1 hypothetical protein -
J3L14_RS28380 (J3L14_28380) 2517498..2517719 - 222 WP_004552390.1 hypothetical protein -
J3L14_RS28385 (J3L14_28385) 2517855..2517986 - 132 WP_009923452.1 bacteriophage protein Gp48 -
J3L14_RS28390 (J3L14_28390) 2517996..2518271 - 276 WP_004552388.1 bacteriophage protein Gp49 -
J3L14_RS28395 (J3L14_28395) 2518891..2519112 + 222 WP_004547086.1 BrnT family toxin Toxin
J3L14_RS28400 (J3L14_28400) 2519096..2519380 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
J3L14_RS28410 (J3L14_28410) 2520027..2520410 + 384 Protein_2091 integrase core domain-containing protein -
J3L14_RS28415 (J3L14_28415) 2520409..2520801 + 393 Protein_2092 IS110 family transposase -
J3L14_RS28420 (J3L14_28420) 2521028..2521144 - 117 Protein_2093 transposase -
J3L14_RS28425 (J3L14_28425) 2521391..2522851 + 1461 WP_004538070.1 metallophosphoesterase -
J3L14_RS28430 (J3L14_28430) 2523010..2524272 + 1263 WP_191044087.1 cytochrome c peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2520114..2520410 296


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(14-65)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 74 a.a.        Molecular weight: 8634.77 Da        Isoelectric Point: 8.4920

>T196871 WP_004547086.1 NZ_CP071762:2518891-2519112 [Burkholderia pseudomallei]
MECRWRLQRNSTGQDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Download         Length: 222 bp

>T196871 NZ_CP095498:1985987-1986403 [Burkholderia cepacia]
GTGACCACGCTCTACATGCTCGATACGAACATCTGTTCGTTCATCATGCGCGAGCGGCCGCCGGTAGTGCTGTCGCGGCT
GCAATCGTGCGTCAATGCGCAGCACCGGATCGTCGTGTCGGCCATCACTTATGCCGAAATGCGGTTCGGCGCAGTCGGCA
GGAAAGCGTCGCCGAAGCATGCGGAACTCGTGACGGCCTTTGTCTCGCGGCTCGATGGCGTGCTGTCGTGGGATACGGCG
GCCGTCGATGCGACGGCCGCGATTCGTGCCGATCTCGCCGCGCGTGGCACGCCGATCGGTGCGAACGATGCGTCGATCGC
CGGGCATGCGATCGCGGCCGGTGCGGTGCTCGTCACGAACAACGGCCGGGAGTTCGGCCGGGTGGCCGGGTTGTCGCTCG
AAGACTGGGCGGCCTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT196871 WP_004525420.1 NZ_CP071762:2519096-2519380 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT196871 NZ_CP095498:1985730-1985990 [Burkholderia cepacia]
ATGCGCACCGTTTCCATTTTCAAGAACGCCCGTAACCAGGCAATCCGCATCCCGAAGGACATGGAGTTCGAGGGGGTGAC
GGAACTCGAGATCCGCCGCGAGGGCGACACACTGATGCTGCGGCCGGTGCGGCCCACGTGGCTCTCGTTCGCGAGCGAAC
CGCTGGCCGACGCCGATTTCCTCGTCGAACGCCCGGCCGTGATCGAGTCCGGCCGCTTCGACCTTTCCGGCGGCGCCGCG
CCGACGGAGTCCGGCCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q63NA2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References