69877

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 2261255..2261696 Replicon chromosome
Accession NZ_CP018374
Organism Burkholderia pseudomallei strain 2002721184

Toxin (Protein)


Gene name brnT Uniprot ID -
Locus tag BOC38_RS32895 Protein ID WP_009939402.1
Coordinates 2261255..2261428 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag BOC38_RS32900 Protein ID WP_004525420.1
Coordinates 2261412..2261696 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOC38_RS32850 2256290..2256697 + 408 WP_076853050.1 DNA mismatch endonuclease Vsr -
BOC38_RS32860 2257673..2258101 - 429 Protein_1828 DDE-type integrase/transposase/recombinase -
BOC38_RS39490 2258099..2258686 - 588 Protein_1829 prohibitin family protein -
BOC38_RS32865 2258737..2259318 + 582 WP_004542548.1 hypothetical protein -
BOC38_RS39495 2259549..2259848 - 300 WP_160458000.1 hypothetical protein -
BOC38_RS32875 2259815..2260036 - 222 WP_004552390.1 hypothetical protein -
BOC38_RS32885 2260172..2260303 - 132 WP_009923452.1 bacteriophage protein Gp48 -
BOC38_RS32890 2260313..2260588 - 276 WP_004537565.1 bacteriophage protein Gp49 -
BOC38_RS32895 2261255..2261428 + 174 WP_009939402.1 BrnT family toxin Toxin
BOC38_RS32900 2261412..2261696 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
BOC38_RS32915 2262343..2262726 + 384 Protein_1837 DDE-type integrase/transposase/recombinase -
BOC38_RS32920 2262689..2263117 + 429 Protein_1838 IS110 family transposase -
BOC38_RS32925 2263344..2263460 - 117 Protein_1839 IS3 family transposase -
BOC38_RS32930 2263726..2265186 + 1461 WP_020850157.1 metallophosphoesterase -
BOC38_RS32935 2265345..2266580 + 1236 WP_009967728.1 c-type cytochrome -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2247605..2262726 15121
flank IS/Tn - - 2262430..2262726 296


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(4-49)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6691.59 Da        Isoelectric Point: 7.9950

>T69877 WP_009939402.1 NZ_CP018374:2261255-2261428 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Download         Length: 174 bp

>T69877 NZ_CP018374:2261255-2261428 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT69877 WP_004525420.1 NZ_CP018374:2261412-2261696 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT69877 NZ_CP018374:2261412-2261696 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAACCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References