114077

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 1557070..1557511 Replicon chromosome
Accession NZ_CP033706
Organism Burkholderia pseudomallei strain FDAARGOS_592

Toxin (Protein)


Gene name brnT Uniprot ID -
Locus tag EGY15_RS28825 Protein ID WP_009939402.1
Coordinates 1557070..1557243 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag EGY15_RS28830 Protein ID WP_004525420.1
Coordinates 1557227..1557511 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY15_RS28775 1552292..1552408 + 117 Protein_1215 transposase -
EGY15_RS28780 1552635..1553063 - 429 Protein_1216 IS110 family transposase -
EGY15_RS28785 1553428..1554201 - 774 Protein_1217 prohibitin family protein -
EGY15_RS37740 1554198..1554506 - 309 WP_172966689.1 hypothetical protein -
EGY15_RS28795 1554598..1555188 + 591 WP_004552393.1 hypothetical protein -
EGY15_RS37745 1555363..1555581 - 219 WP_004552392.1 hypothetical protein -
EGY15_RS28805 1555629..1555850 - 222 WP_004552390.1 hypothetical protein -
EGY15_RS28820 1556127..1556402 - 276 WP_004552388.1 bacteriophage protein Gp49 -
EGY15_RS28825 1557070..1557243 + 174 WP_009939402.1 BrnT family toxin Toxin
EGY15_RS28830 1557227..1557511 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
EGY15_RS28845 1558157..1558564 + 408 Protein_1225 DDE-type integrase/transposase/recombinase -
EGY15_RS28850 1559182..1559679 + 498 WP_004552308.1 hypothetical protein -
EGY15_RS28860 1560108..1560524 - 417 WP_004552310.1 type II toxin-antitoxin system HicB family antitoxin -
EGY15_RS28865 1560569..1560748 - 180 WP_009893919.1 type II toxin-antitoxin system HicA family toxin -
EGY15_RS28870 1561028..1561555 + 528 WP_004552312.1 PAAR domain-containing protein -
EGY15_RS28875 1561552..1562286 + 735 WP_004552313.1 VRR-NUC domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1546940..1580279 33339
flank IS/Tn - - 1558244..1558564 320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(4-49)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6691.59 Da        Isoelectric Point: 7.9950

>T114077 WP_009939402.1 NZ_CP033706:1557070-1557243 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Download         Length: 174 bp

>T114077 NZ_CP033706:1557070-1557243 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT114077 WP_004525420.1 NZ_CP033706:1557227-1557511 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT114077 NZ_CP033706:1557227-1557511 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGTTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References