Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 3210900..3211540 Replicon chromosome
Accession NZ_CP046583
Organism Burkholderia pseudomallei strain B03

Toxin (Protein)


Gene name hicA Uniprot ID Q2T3Q6
Locus tag GN245_RS14540 Protein ID WP_009893919.1
Coordinates 3211361..3211540 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q63NA5
Locus tag GN245_RS14535 Protein ID WP_004552310.1
Coordinates 3210900..3211316 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GN245_RS14495 3206088..3206219 + 132 WP_009923452.1 bacteriophage protein Gp48 -
GN245_RS14505 3206355..3206576 + 222 WP_038759295.1 hypothetical protein -
GN245_RS34390 3206543..3206842 + 300 WP_161788725.1 hypothetical protein -
GN245_RS14515 3207073..3207654 - 582 Protein_2621 hypothetical protein -
GN245_RS14520 3207910..3208059 + 150 WP_004537646.1 bacteriophage protein Gp44 -
GN245_RS34395 3208056..3208829 + 774 Protein_2623 prohibitin family protein -
GN245_RS34400 3208827..3209255 + 429 Protein_2624 DDE-type integrase/transposase/recombinase -
GN245_RS14530 3209974..3210471 + 498 WP_004552308.1 hypothetical protein -
GN245_RS14535 3210900..3211316 - 417 WP_004552310.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GN245_RS14540 3211361..3211540 - 180 WP_009893919.1 type II toxin-antitoxin system HicA family toxin Toxin
GN245_RS14545 3211820..3212053 + 234 Protein_2628 PAAR domain-containing protein -
GN245_RS14550 3212287..3212814 + 528 WP_004552312.1 PAAR domain-containing protein -
GN245_RS14555 3212811..3213545 + 735 WP_004552313.1 VRR-NUC domain-containing protein -
GN245_RS14560 3213555..3214670 + 1116 WP_009893924.1 DUF3396 domain-containing protein -
GN245_RS14565 3214741..3215316 - 576 WP_004552315.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3197583..3231163 33580


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-126)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6618.82 Da        Isoelectric Point: 11.2336

>T144546 WP_009893919.1 NZ_CP046583:c3211540-3211361 [Burkholderia pseudomallei]
MNSSKLIRMLEEDGWRLVRVTGSHHHFKHPKKPGLVTVPHPKKDLPIGTVKSIQKSAGL

Download         Length: 180 bp

>T144546 NZ_CP062146:c3800787-3800368 [Proteus mirabilis]
GTGAAAAAAGTCTATATGCTTGATACAAACATTTGTTCTTTTATCATGCGAGAGCAACCTATTTCTTTGCTCGAAAAACT
TCAGAAATGTGTAATGAACCACGATACTATTGTCATATCAGCGATTACTTATTCAGAGATGCGCTTTGGAGCAATAGGAA
AAAAAGCATCACCTAAACACAATCGTTTGGTTGATGCTTTCTGTGAACGCGTTGACGCGATTTTGGCATGGGATAAAGCG
GCGGTAGACGCAACTACCGTGATAAAAAAATGCTTATCTGATGTGGGATTGCCCATAGGAAATAATGATAGCGCGATTGC
TGGTCATGCTGTCGCGGTTAATGCAATATTGGTAACAAATAATACCCGTGAATTTTCACGAGTTGAAGGACTAAAAATAG
AGGACTGGACTCATGTTTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15120.06 Da        Isoelectric Point: 4.7025

>AT144546 WP_004552310.1 NZ_CP046583:c3211316-3210900 [Burkholderia pseudomallei]
MEFPIAVHKDDGSVYGVTVPDIPGVHSWGETIDDAIKNTREAIVGHVETLIELGEDVEFTCSTVEELVAKPEYAGAVWAL
VSVDLSQLDSKPERINVSIPRFVLHKIDAYVASRHETRSGFLARAALEALNEGKVRHA

Download         Length: 417 bp

>AT144546 NZ_CP062146:c3801017-3800784 [Proteus mirabilis]
ATGAGAACGAGAACTGTAGCTGTATTTAAAAACGGAAGTAACCGAGCCATTCGCTTACCTAAAGATTTCGATTTTGATGG
TGTCAATGAACTCGAGATAAGCCGAGATGGGGATTGTATTATTTTACGCCCTGTAAAACCTAACTGGATTTCATTACAAA
ACGAAGAAAAAGCCGACAGTGATTTCTTGCTTGAACGCGATGATGTTATTGAAGAAGGACGAGTTATTCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4C26
PDB 6G26

Antitoxin

Source ID Structure
PDB 6G1C
PDB 6G1N
PDB 6G26
AlphaFold DB Q63NA5

References