Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1560108..1560748 Replicon chromosome
Accession NZ_CP033706
Organism Burkholderia pseudomallei strain FDAARGOS_592

Toxin (Protein)


Gene name hicA Uniprot ID Q2T3Q6
Locus tag EGY15_RS28865 Protein ID WP_009893919.1
Coordinates 1560569..1560748 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q63NA5
Locus tag EGY15_RS28860 Protein ID WP_004552310.1
Coordinates 1560108..1560524 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY15_RS37745 1555363..1555581 - 219 WP_004552392.1 hypothetical protein -
EGY15_RS28805 1555629..1555850 - 222 WP_004552390.1 hypothetical protein -
EGY15_RS28820 1556127..1556402 - 276 WP_004552388.1 bacteriophage protein Gp49 -
EGY15_RS28825 1557070..1557243 + 174 WP_009939402.1 BrnT family toxin -
EGY15_RS28830 1557227..1557511 + 285 WP_004525420.1 BrnA antitoxin family protein -
EGY15_RS28845 1558157..1558564 + 408 Protein_1225 DDE-type integrase/transposase/recombinase -
EGY15_RS28850 1559182..1559679 + 498 WP_004552308.1 hypothetical protein -
EGY15_RS28860 1560108..1560524 - 417 WP_004552310.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EGY15_RS28865 1560569..1560748 - 180 WP_009893919.1 type II toxin-antitoxin system HicA family toxin Toxin
EGY15_RS28870 1561028..1561555 + 528 WP_004552312.1 PAAR domain-containing protein -
EGY15_RS28875 1561552..1562286 + 735 WP_004552313.1 VRR-NUC domain-containing protein -
EGY15_RS28880 1562296..1563411 + 1116 WP_004552314.1 DUF3396 domain-containing protein -
EGY15_RS28885 1563482..1564057 - 576 WP_004552315.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1546940..1580279 33339
flank IS/Tn - - 1558244..1558564 320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-126)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6618.82 Da        Isoelectric Point: 11.2336

>T114078 WP_009893919.1 NZ_CP033706:c1560748-1560569 [Burkholderia pseudomallei]
MNSSKLIRMLEEDGWRLVRVTGSHHHFKHPKKPGLVTVPHPKKDLPIGTVKSIQKSAGL

Download         Length: 180 bp

>T114078 NZ_CP033706:c1560748-1560569 [Burkholderia pseudomallei]
ATGAACTCATCGAAGCTGATCCGGATGCTTGAGGAAGATGGCTGGAGGTTGGTTCGAGTGACAGGCAGCCATCATCATTT
CAAACACCCCAAGAAGCCAGGCCTTGTGACGGTTCCCCACCCGAAGAAGGACCTACCGATTGGGACTGTAAAAAGCATCC
AGAAATCCGCCGGCCTGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15120.06 Da        Isoelectric Point: 4.7025

>AT114078 WP_004552310.1 NZ_CP033706:c1560524-1560108 [Burkholderia pseudomallei]
MEFPIAVHKDDGSVYGVTVPDIPGVHSWGETIDDAIKNTREAIVGHVETLIELGEDVEFTCSTVEELVAKPEYAGAVWAL
VSVDLSQLDSKPERINVSIPRFVLHKIDAYVASRHETRSGFLARAALEALNEGKVRHA

Download         Length: 417 bp

>AT114078 NZ_CP033706:c1560524-1560108 [Burkholderia pseudomallei]
ATGGAATTTCCCATCGCAGTGCATAAGGACGATGGGAGCGTCTACGGCGTGACGGTCCCGGATATTCCGGGCGTTCACTC
CTGGGGCGAAACCATCGATGATGCAATCAAAAACACGAGAGAGGCTATCGTCGGCCATGTAGAGACATTGATCGAGCTTG
GAGAAGATGTTGAATTCACTTGCTCGACGGTGGAAGAACTGGTCGCAAAGCCCGAGTACGCCGGGGCGGTTTGGGCGCTC
GTCAGCGTTGATCTTTCTCAGCTTGATTCCAAGCCCGAGCGGATCAATGTGAGTATCCCTCGCTTCGTGCTGCACAAGAT
TGACGCCTATGTGGCATCCCGCCACGAGACTAGAAGCGGATTTCTTGCGCGTGCTGCCCTGGAGGCACTTAATGAAGGCA
AAGTTAGGCACGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4C26
PDB 6G26

Antitoxin

Source ID Structure
PDB 6G1C
PDB 6G1N
PDB 6G26
AlphaFold DB Q63NA5

References