120012

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 517441..517882 Replicon chromosome
Accession NZ_CP037757
Organism Burkholderia pseudomallei strain BPs115

Toxin (Protein)


Gene name brnT Uniprot ID -
Locus tag EYA82_RS02730 Protein ID WP_009939402.1
Coordinates 517709..517882 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag EYA82_RS02725 Protein ID WP_004525420.1
Coordinates 517441..517725 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EYA82_RS02695 513805..514125 + 321 WP_004196029.1 DUF4212 domain-containing protein -
EYA82_RS02700 514119..516134 + 2016 WP_024431205.1 cation acetate symporter -
EYA82_RS02710 516388..516795 - 408 Protein_420 DDE-type integrase/transposase/recombinase -
EYA82_RS02725 517441..517725 - 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
EYA82_RS02730 517709..517882 - 174 WP_009939402.1 BrnT family toxin Toxin
EYA82_RS02735 518550..518825 + 276 WP_004552388.1 bacteriophage protein Gp49 -
EYA82_RS02740 518835..518966 + 132 WP_009923452.1 bacteriophage protein Gp48 -
EYA82_RS02750 519102..519275 + 174 WP_132108117.1 hypothetical protein -
EYA82_RS36285 519323..519541 + 219 WP_004552392.1 hypothetical protein -
EYA82_RS02760 519914..520063 + 150 WP_004537646.1 bacteriophage protein Gp44 -
EYA82_RS02765 520060..520833 + 774 Protein_428 prohibitin family protein -
EYA82_RS37120 520831..521235 + 405 Protein_429 DDE-type integrase/transposase/recombinase -
EYA82_RS02770 521198..521626 + 429 Protein_430 IS110 family transposase -
EYA82_RS02775 521853..521969 - 117 Protein_431 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 514119..527257 13138
flank IS/Tn - - 516388..516708 320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(4-49)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6691.59 Da        Isoelectric Point: 7.9950

>T120012 WP_009939402.1 NZ_CP037757:c517882-517709 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Download         Length: 174 bp

>T120012 NZ_CP037757:c517882-517709 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT120012 WP_004525420.1 NZ_CP037757:c517725-517441 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT120012 NZ_CP037757:c517725-517441 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGTTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References