Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1587647..1588287 Replicon chromosome
Accession NZ_CP018401
Organism Burkholderia pseudomallei strain 2013746811

Toxin (Protein)


Gene name hicA Uniprot ID Q2T3Q6
Locus tag BOC51_RS29450 Protein ID WP_009893919.1
Coordinates 1587647..1587826 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q63NA5
Locus tag BOC51_RS29455 Protein ID WP_004552310.1
Coordinates 1587871..1588287 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOC51_RS29430 1584341..1584916 + 576 WP_004552315.1 recombinase family protein -
BOC51_RS29435 1584987..1586101 - 1115 Protein_1353 DUF3396 domain-containing protein -
BOC51_RS29440 1586098..1586844 - 747 WP_151287559.1 VRR-NUC domain-containing protein -
BOC51_RS29445 1586841..1587368 - 528 WP_004552312.1 PAAR domain-containing protein -
BOC51_RS29450 1587647..1587826 + 180 WP_009893919.1 type II toxin-antitoxin system HicA family toxin Toxin
BOC51_RS29455 1587871..1588287 + 417 WP_004552310.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BOC51_RS29465 1588716..1589213 - 498 WP_004552308.1 hypothetical protein -
BOC51_RS29470 1589831..1590238 - 408 Protein_1359 DDE-type integrase/transposase/recombinase -
BOC51_RS29485 1590884..1591168 - 285 WP_004525420.1 BrnA antitoxin family protein -
BOC51_RS29490 1591152..1591325 - 174 WP_009939402.1 BrnT family toxin -
BOC51_RS29495 1591993..1592268 + 276 WP_025988352.1 hypothetical protein -
BOC51_RS29500 1592278..1592409 + 132 WP_009923452.1 bacteriophage protein Gp48 -
BOC51_RS29510 1592545..1592766 + 222 WP_004525418.1 hypothetical protein -
BOC51_RS38125 1592814..1593032 + 219 WP_004552392.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1568500..1601453 32953
flank IS/Tn - - 1589831..1590151 320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-126)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6618.82 Da        Isoelectric Point: 11.2336

>T69904 WP_009893919.1 NZ_CP018401:1587647-1587826 [Burkholderia pseudomallei]
MNSSKLIRMLEEDGWRLVRVTGSHHHFKHPKKPGLVTVPHPKKDLPIGTVKSIQKSAGL

Download         Length: 180 bp

>T69904 NZ_CP018401:1587647-1587826 [Burkholderia pseudomallei]
ATGAACTCATCGAAGCTGATCCGGATGCTTGAGGAAGATGGCTGGAGGTTGGTTCGAGTGACAGGCAGCCATCATCATTT
CAAACACCCCAAGAAGCCAGGCCTTGTGACGGTTCCCCACCCGAAGAAGGACCTACCGATTGGGACTGTAAAAAGCATCC
AGAAATCCGCCGGCCTGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15120.06 Da        Isoelectric Point: 4.7025

>AT69904 WP_004552310.1 NZ_CP018401:1587871-1588287 [Burkholderia pseudomallei]
MEFPIAVHKDDGSVYGVTVPDIPGVHSWGETIDDAIKNTREAIVGHVETLIELGEDVEFTCSTVEELVAKPEYAGAVWAL
VSVDLSQLDSKPERINVSIPRFVLHKIDAYVASRHETRSGFLARAALEALNEGKVRHA

Download         Length: 417 bp

>AT69904 NZ_CP018401:1587871-1588287 [Burkholderia pseudomallei]
ATGGAATTTCCCATCGCAGTGCATAAGGACGATGGGAGCGTCTACGGCGTGACGGTCCCGGATATTCCGGGCGTTCACTC
CTGGGGCGAAACCATCGATGATGCAATCAAAAACACGAGAGAGGCTATCGTCGGCCATGTAGAGACATTGATCGAGCTTG
GAGAAGATGTTGAATTCACTTGCTCGACGGTGGAAGAACTGGTCGCAAAGCCCGAGTACGCCGGGGCGGTTTGGGCGCTC
GTCAGCGTTGATCTTTCTCAGCTTGATTCCAAGCCCGAGCGGATCAATGTGAGTATCCCTCGCTTCGTGCTGCACAAGAT
TGACGCCTATGTGGCATCCCGCCACGAGACTAGAAGCGGATTTCTTGCGCGTGCTGCCCTGGAGGCACTTAATGAAGGCA
AAGTTAGGCACGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4C26
PDB 6G26

Antitoxin

Source ID Structure
PDB 6G1C
PDB 6G1N
PDB 6G26
AlphaFold DB Q63NA5

References