65433

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 577006..577543 Replicon chromosome
Accession NZ_CP016912
Organism Burkholderia pseudomallei strain Burk178-Type2

Toxin (Protein)


Gene name brnT Uniprot ID Q2T6G2
Locus tag BFR06_RS23755 Protein ID WP_004549731.1
Coordinates 577006..577275 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag BFR06_RS23760 Protein ID WP_004525420.1
Coordinates 577259..577543 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFR06_RS40875 572982..573410 - 429 Protein_450 DDE-type integrase/transposase/recombinase -
BFR06_RS40880 573408..574181 - 774 Protein_451 prohibitin family protein -
BFR06_RS23725 574178..574327 - 150 WP_043276559.1 hypothetical protein -
BFR06_RS23730 574573..575228 + 656 Protein_453 hypothetical protein -
BFR06_RS40885 575414..575632 - 219 WP_004552392.1 hypothetical protein -
BFR06_RS23735 575680..575901 - 222 WP_024430861.1 hypothetical protein -
BFR06_RS23750 576178..576453 - 276 WP_038726742.1 hypothetical protein -
BFR06_RS23755 577006..577275 + 270 WP_004549731.1 BrnT family toxin Toxin
BFR06_RS23760 577259..577543 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
BFR06_RS23775 578191..578574 + 384 Protein_459 DDE-type integrase/transposase/recombinase -
BFR06_RS23780 578537..578965 + 429 Protein_460 IS110 family transposase -
BFR06_RS23785 579191..579307 - 117 Protein_461 IS3 family transposase -
BFR06_RS23790 579554..581014 + 1461 WP_004547073.1 metallophosphoesterase -
BFR06_RS23795 581173..582435 + 1263 WP_004530738.1 c-type cytochrome -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 572982..578574 5592
flank IS/Tn - - 578278..578574 296


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10157.46 Da        Isoelectric Point: 5.6831

>T65433 WP_004549731.1 NZ_CP016912:577006-577275 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T65433 NZ_CP016912:577006-577275 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT65433 WP_004525420.1 NZ_CP016912:577259-577543 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT65433 NZ_CP016912:577259-577543 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q2T6G2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References