65427

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 577007..577544 Replicon chromosome
Accession NZ_CP016910
Organism Burkholderia pseudomallei strain Burk178-Type1

Toxin (Protein)


Gene name brnT Uniprot ID Q2T6G2
Locus tag BFR05_RS23750 Protein ID WP_004549731.1
Coordinates 577007..577276 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag BFR05_RS23755 Protein ID WP_004525420.1
Coordinates 577260..577544 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFR05_RS40870 572983..573411 - 429 Protein_450 DDE-type integrase/transposase/recombinase -
BFR05_RS40875 573409..574182 - 774 Protein_451 prohibitin family protein -
BFR05_RS23720 574179..574328 - 150 WP_043276559.1 hypothetical protein -
BFR05_RS23725 574574..575229 + 656 Protein_453 hypothetical protein -
BFR05_RS40880 575415..575633 - 219 WP_004552392.1 hypothetical protein -
BFR05_RS23730 575681..575902 - 222 WP_024430861.1 hypothetical protein -
BFR05_RS23745 576179..576454 - 276 WP_038726742.1 hypothetical protein -
BFR05_RS23750 577007..577276 + 270 WP_004549731.1 BrnT family toxin Toxin
BFR05_RS23755 577260..577544 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
BFR05_RS23770 578192..578575 + 384 Protein_459 DDE-type integrase/transposase/recombinase -
BFR05_RS23775 578538..578966 + 429 Protein_460 IS110 family transposase -
BFR05_RS23780 579192..579308 - 117 Protein_461 IS3 family transposase -
BFR05_RS23785 579555..581015 + 1461 WP_004547073.1 metallophosphoesterase -
BFR05_RS23790 581174..582436 + 1263 WP_004530738.1 c-type cytochrome -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 572983..578575 5592
flank IS/Tn - - 578279..578575 296


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10157.46 Da        Isoelectric Point: 5.6831

>T65427 WP_004549731.1 NZ_CP016910:577007-577276 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T65427 NZ_CP016910:577007-577276 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT65427 WP_004525420.1 NZ_CP016910:577260-577544 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT65427 NZ_CP016910:577260-577544 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q2T6G2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References