49224

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 2336171..2336612 Replicon chromosome
Accession NZ_CP009537
Organism Burkholderia pseudomallei K96243

Toxin (Protein)


Gene name brnT Uniprot ID -
Locus tag AQ15_RS09620 Protein ID WP_009939402.1
Coordinates 2336439..2336612 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag AQ15_RS09615 Protein ID WP_004525420.1
Coordinates 2336171..2336455 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AQ15_RS09580 2331396..2332130 - 735 WP_004552313.1 VRR-NUC domain-containing protein -
AQ15_RS09585 2332127..2332654 - 528 WP_004552312.1 PAAR domain-containing protein -
AQ15_RS09590 2332934..2333113 + 180 WP_009893919.1 type II toxin-antitoxin system HicA family toxin -
AQ15_RS09595 2333158..2333574 + 417 WP_004552310.1 type II toxin-antitoxin system HicB family antitoxin -
AQ15_RS09600 2334003..2334500 - 498 WP_004552308.1 hypothetical protein -
AQ15_RS34215 2335118..2335525 - 408 Protein_1927 DDE-type integrase/transposase/recombinase -
AQ15_RS09615 2336171..2336455 - 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
AQ15_RS09620 2336439..2336612 - 174 WP_009939402.1 BrnT family toxin Toxin
AQ15_RS09625 2337280..2337555 + 276 WP_004552388.1 bacteriophage protein Gp49 -
AQ15_RS09630 2337565..2337696 + 132 WP_009923452.1 bacteriophage protein Gp48 -
AQ15_RS09640 2337832..2338053 + 222 WP_004552390.1 hypothetical protein -
AQ15_RS40335 2338020..2338319 + 300 WP_160469016.1 hypothetical protein -
AQ15_RS09650 2338494..2339084 - 591 WP_038717720.1 hypothetical protein -
AQ15_RS09655 2339334..2339483 + 150 WP_004537646.1 bacteriophage protein Gp44 -
AQ15_RS09660 2339480..2340253 + 774 Protein_1936 prohibitin family protein -
AQ15_RS40875 2340251..2340655 + 405 Protein_1937 DDE-type integrase/transposase/recombinase -
AQ15_RS34230 2340618..2341046 + 429 Protein_1938 IS110 family transposase -
AQ15_RS09665 2341273..2341389 - 117 Protein_1939 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2313777..2343096 29319
flank IS/Tn - - 2335118..2335438 320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(4-49)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6691.59 Da        Isoelectric Point: 7.9950

>T49224 WP_009939402.1 NZ_CP009537:c2336612-2336439 [Burkholderia pseudomallei K96243]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Download         Length: 174 bp

>T49224 NZ_CP009537:c2336612-2336439 [Burkholderia pseudomallei K96243]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT49224 WP_004525420.1 NZ_CP009537:c2336455-2336171 [Burkholderia pseudomallei K96243]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT49224 NZ_CP009537:c2336455-2336171 [Burkholderia pseudomallei K96243]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGTTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References