48467

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 411364..411901 Replicon chromosome
Accession NZ_CP009150
Organism Burkholderia pseudomallei B03

Toxin (Protein)


Gene name brnT Uniprot ID Q2T6G2
Locus tag X995_RS01680 Protein ID WP_004549731.1
Coordinates 411364..411633 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag X995_RS01685 Protein ID WP_004525420.1
Coordinates 411617..411901 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
X995_RS41065 407341..407769 - 429 Protein_320 DDE-type integrase/transposase/recombinase -
X995_RS40835 407767..408540 - 774 Protein_321 prohibitin family protein -
X995_RS01645 408537..408686 - 150 WP_004537646.1 bacteriophage protein Gp44 -
X995_RS01650 408942..409523 + 582 WP_004542548.1 hypothetical protein -
X995_RS40250 409754..410053 - 300 WP_161788725.1 hypothetical protein -
X995_RS01660 410020..410241 - 222 WP_038759295.1 hypothetical protein -
X995_RS41070 410377..410508 - 132 WP_009923452.1 bacteriophage protein Gp48 -
X995_RS01675 410518..410793 - 276 WP_038759296.1 hypothetical protein -
X995_RS01680 411364..411633 + 270 WP_004549731.1 BrnT family toxin Toxin
X995_RS01685 411617..411901 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
X995_RS01695 412548..412931 + 384 Protein_330 DDE-type integrase/transposase/recombinase -
X995_RS31765 412894..413322 + 429 Protein_331 IS110 family transposase -
X995_RS01700 413549..413665 - 117 Protein_332 IS3 family transposase -
X995_RS01705 413912..415372 + 1461 WP_044583952.1 metallophosphoesterase -
X995_RS01710 415531..416793 + 1263 WP_004530738.1 c-type cytochrome -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 385433..415372 29939


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10157.46 Da        Isoelectric Point: 5.6831

>T48467 WP_004549731.1 NZ_CP009150:411364-411633 [Burkholderia pseudomallei B03]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T48467 NZ_CP009150:411364-411633 [Burkholderia pseudomallei B03]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT48467 WP_004525420.1 NZ_CP009150:411617-411901 [Burkholderia pseudomallei B03]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT48467 NZ_CP009150:411617-411901 [Burkholderia pseudomallei B03]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q2T6G2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References