120593

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 511839..512280 Replicon chromosome
Accession NZ_CP037973
Organism Burkholderia pseudomallei strain BPs114

Toxin (Protein)


Gene name brnT Uniprot ID -
Locus tag E2R28_RS02715 Protein ID WP_009939402.1
Coordinates 512107..512280 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag E2R28_RS02710 Protein ID WP_004525420.1
Coordinates 511839..512123 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2R28_RS02670 506853..507173 + 321 WP_004196029.1 DUF4212 domain-containing protein -
E2R28_RS02675 507167..509182 + 2016 WP_004525415.1 cation acetate symporter -
E2R28_RS36185 509406..509861 - 456 WP_166676783.1 toprim domain-containing protein -
E2R28_RS36190 509759..510323 - 565 Protein_422 PriCT-2 domain-containing protein -
E2R28_RS02690 510500..510683 + 184 Protein_423 recombinase family protein -
E2R28_RS02695 510786..511193 - 408 Protein_424 DDE-type integrase/transposase/recombinase -
E2R28_RS02710 511839..512123 - 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
E2R28_RS02715 512107..512280 - 174 WP_009939402.1 BrnT family toxin Toxin
E2R28_RS02720 512948..513223 + 276 WP_004552388.1 bacteriophage protein Gp49 -
E2R28_RS02725 513233..513364 + 132 WP_009923452.1 bacteriophage protein Gp48 -
E2R28_RS02735 513500..513721 + 222 WP_004538072.1 hypothetical protein -
E2R28_RS36195 513769..513987 + 219 WP_004552392.1 hypothetical protein -
E2R28_RS02745 514361..514510 + 150 WP_004537646.1 bacteriophage protein Gp44 -
E2R28_RS02750 514507..515280 + 774 Protein_432 prohibitin family protein -
E2R28_RS37050 515278..515682 + 405 Protein_433 DDE-type integrase/transposase/recombinase -
E2R28_RS02755 515645..516073 + 429 Protein_434 IS110 family transposase -
E2R28_RS02760 516300..516416 - 117 Protein_435 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 507167..518075 10908
flank IS/Tn - - 510786..511106 320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(4-49)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6691.59 Da        Isoelectric Point: 7.9950

>T120593 WP_009939402.1 NZ_CP037973:c512280-512107 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Download         Length: 174 bp

>T120593 NZ_CP037973:c512280-512107 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT120593 WP_004525420.1 NZ_CP037973:c512123-511839 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT120593 NZ_CP037973:c512123-511839 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References