49218

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 1432540..1432981 Replicon chromosome
Accession NZ_CP009536
Organism Burkholderia pseudomallei 7894

Toxin (Protein)


Gene name brnT Uniprot ID -
Locus tag BG97_RS24320 Protein ID WP_009939402.1
Coordinates 1432540..1432713 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag BG97_RS24325 Protein ID WP_004525420.1
Coordinates 1432697..1432981 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG97_RS37520 1427575..1427982 + 408 WP_076853050.1 DNA mismatch endonuclease Vsr -
BG97_RS24275 1428958..1429386 - 429 Protein_1197 DDE-type integrase/transposase/recombinase -
BG97_RS41900 1429384..1429971 - 588 Protein_1198 prohibitin family protein -
BG97_RS24285 1430022..1430603 + 582 WP_004542548.1 hypothetical protein -
BG97_RS41445 1430834..1431133 - 300 WP_160458000.1 hypothetical protein -
BG97_RS24295 1431100..1431321 - 222 WP_004552390.1 hypothetical protein -
BG97_RS24305 1431457..1431588 - 132 WP_009923452.1 bacteriophage protein Gp48 -
BG97_RS24310 1431598..1431873 - 276 WP_004537565.1 bacteriophage protein Gp49 -
BG97_RS24320 1432540..1432713 + 174 WP_009939402.1 BrnT family toxin Toxin
BG97_RS24325 1432697..1432981 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
BG97_RS24335 1433628..1434011 + 384 Protein_1206 DDE-type integrase/transposase/recombinase -
BG97_RS37540 1433974..1434402 + 429 Protein_1207 IS110 family transposase -
BG97_RS24340 1434629..1434745 - 117 Protein_1208 IS3 family transposase -
BG97_RS24345 1435011..1436471 + 1461 WP_020850157.1 metallophosphoesterase -
BG97_RS24350 1436630..1437865 + 1236 WP_009967728.1 c-type cytochrome -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1418890..1434011 15121
flank IS/Tn - - 1433715..1434011 296


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(4-49)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6691.59 Da        Isoelectric Point: 7.9950

>T49218 WP_009939402.1 NZ_CP009536:1432540-1432713 [Burkholderia pseudomallei 7894]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Download         Length: 174 bp

>T49218 NZ_CP009536:1432540-1432713 [Burkholderia pseudomallei 7894]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT49218 WP_004525420.1 NZ_CP009536:1432697-1432981 [Burkholderia pseudomallei 7894]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT49218 NZ_CP009536:1432697-1432981 [Burkholderia pseudomallei 7894]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAACCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References