47433

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 1546374..1546911 Replicon chromosome
Accession NZ_CP008783
Organism Burkholderia pseudomallei MSHR5855

Toxin (Protein)


Gene name brnT Uniprot ID Q2T6G2
Locus tag DP63_RS06320 Protein ID WP_004549731.1
Coordinates 1546374..1546643 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag DP63_RS06325 Protein ID WP_004525420.1
Coordinates 1546627..1546911 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DP63_RS41295 1542350..1542778 - 429 Protein_1260 DDE-type integrase/transposase/recombinase -
DP63_RS41300 1542776..1543549 - 774 Protein_1261 prohibitin family protein -
DP63_RS41305 1543546..1543695 - 150 WP_043276559.1 hypothetical protein -
DP63_RS06295 1543941..1544596 + 656 Protein_1263 hypothetical protein -
DP63_RS40725 1544782..1545000 - 219 WP_004552392.1 hypothetical protein -
DP63_RS06305 1545048..1545269 - 222 WP_024430861.1 hypothetical protein -
DP63_RS33590 1545546..1545821 - 276 WP_038726742.1 hypothetical protein -
DP63_RS06320 1546374..1546643 + 270 WP_004549731.1 BrnT family toxin Toxin
DP63_RS06325 1546627..1546911 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
DP63_RS06335 1547559..1547942 + 384 Protein_1269 DDE-type integrase/transposase/recombinase -
DP63_RS33605 1547905..1548333 + 429 Protein_1270 IS110 family transposase -
DP63_RS33610 1548559..1548675 - 117 Protein_1271 IS3 family transposase -
DP63_RS06340 1548922..1550382 + 1461 WP_004547073.1 metallophosphoesterase -
DP63_RS06345 1550541..1551803 + 1263 WP_004530738.1 c-type cytochrome -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1542350..1547942 5592
flank IS/Tn - - 1547646..1547942 296


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10157.46 Da        Isoelectric Point: 5.6831

>T47433 WP_004549731.1 NZ_CP008783:1546374-1546643 [Burkholderia pseudomallei MSHR5855]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T47433 NZ_CP008783:1546374-1546643 [Burkholderia pseudomallei MSHR5855]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT47433 WP_004525420.1 NZ_CP008783:1546627-1546911 [Burkholderia pseudomallei MSHR5855]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT47433 NZ_CP008783:1546627-1546911 [Burkholderia pseudomallei MSHR5855]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q2T6G2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References