48503

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 1613082..1613619 Replicon chromosome
Accession NZ_CP009164
Organism Burkholderia pseudomallei A79A

Toxin (Protein)


Gene name brnT Uniprot ID Q2T6G2
Locus tag X996_RS06420 Protein ID WP_004549731.1
Coordinates 1613350..1613619 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag X996_RS06415 Protein ID WP_004525420.1
Coordinates 1613082..1613366 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
X996_RS06390 1608190..1609452 - 1263 WP_004530738.1 c-type cytochrome -
X996_RS06395 1609611..1611071 - 1461 WP_044583952.1 metallophosphoesterase -
X996_RS06400 1611318..1611434 + 117 Protein_1267 IS3 family transposase -
X996_RS33260 1611661..1612089 - 429 Protein_1268 IS110 family transposase -
X996_RS06405 1612052..1612435 - 384 Protein_1269 DDE-type integrase/transposase/recombinase -
X996_RS06415 1613082..1613366 - 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
X996_RS06420 1613350..1613619 - 270 WP_004549731.1 BrnT family toxin Toxin
X996_RS06425 1614190..1614465 + 276 WP_038759296.1 hypothetical protein -
X996_RS41130 1614475..1614606 + 132 WP_009923452.1 bacteriophage protein Gp48 -
X996_RS06440 1614742..1614963 + 222 WP_038759295.1 hypothetical protein -
X996_RS40400 1614930..1615229 + 300 WP_161788725.1 hypothetical protein -
X996_RS06450 1615460..1616041 - 582 WP_004542548.1 hypothetical protein -
X996_RS06455 1616297..1616446 + 150 WP_004537646.1 bacteriophage protein Gp44 -
X996_RS40900 1616443..1617216 + 774 Protein_1278 prohibitin family protein -
X996_RS41135 1617214..1617642 + 429 Protein_1279 DDE-type integrase/transposase/recombinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1605968..1639550 33582


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10157.46 Da        Isoelectric Point: 5.6831

>T48503 WP_004549731.1 NZ_CP009164:c1613619-1613350 [Burkholderia pseudomallei A79A]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T48503 NZ_CP009164:c1613619-1613350 [Burkholderia pseudomallei A79A]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT48503 WP_004525420.1 NZ_CP009164:c1613366-1613082 [Burkholderia pseudomallei A79A]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT48503 NZ_CP009164:c1613366-1613082 [Burkholderia pseudomallei A79A]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q2T6G2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References