10016

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type VII Classification (family/domain) TglT-TakA/-
Location 1167053..1168554 Replicon chromosome
Accession NC_000962
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name tglT Uniprot ID P96356
Locus tag Rv1045 Protein ID NP_215561.1
Coordinates 1167673..1168554 (+) Length 294 a.a.

Antitoxin (Protein)


Gene name takA Uniprot ID P96357
Locus tag Rv1044 Protein ID NP_215560.1
Coordinates 1167053..1167676 (+) Length 208 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Rv1040c 1162549..1163376 - 828 YP_177779.1 PE family protein PE8 -
Rv1041c 1164572..1165435 - 864 NP_215557.1 IS2-like transposase -
Rv1042c 1165092..1165499 - 408 NP_215558.1 IS2-like transposase -
Rv1043c 1165781..1166806 - 1026 NP_215559.1 hypothetical protein -
Rv1044 1167053..1167676 + 624 NP_215560.1 hypothetical protein Antitoxin
Rv1045 1167673..1168554 + 882 NP_215561.1 hypothetical protein Toxin
Rv1047 1169423..1170670 + 1248 NP_215563.1 transposase -
Rv1048c 1171038..1172153 - 1116 NP_215564.1 hypothetical protein -
Rv1049 1172386..1172832 + 447 NP_215565.1 transcriptional repressor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside IScluster/Tn - esxN 1158918..1170670 11752


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(49-279)

Antitoxin

Transcriptional regulator, AbiEi antitoxin

(18-66)


Sequences

Toxin        


Download         Length: 294 a.a.        Molecular weight: 31958.65 Da        Isoelectric Point: 6.3901

>T10016 NP_215561.1 NC_000962:1167673-1168554 [Mycobacterium tuberculosis H37Rv]
VTKPYSSPPTNLRSLRDRLTQVAERQGVVFGRLQRHVAMIVVAQFAATLTDDTGAPLLLVKGGSSLELRRGIPDSRTSKD
FDTVARRDIELIHEQLADAGETGWEGFTAIFTAPEEIDVPGMPVKPRRFTAKLSYRGRAFATVPIEVSSVEAGNADQFDT
LTSDALGLVGVPAAVAVPCMTIPWQIAQKLHAVTAVLEEPKVNDRAHDLVDLQLLEGLLLDADLMPTRSACIAIFEARAQ
HPWPPRVATLPHWPLIYAGALEGLDHLELARTVDAAAQAVQRFVARIDRATKR

Download         Length: 882 bp

>T10016 NC_000962:1167673-1168554 [Mycobacterium tuberculosis H37Rv]
GTGACCAAGCCCTATTCGTCGCCGCCAACGAACCTGCGCTCACTACGAGATCGGCTCACCCAAGTAGCGGAACGGCAAGG
TGTCGTGTTCGGTCGACTGCAGCGGCATGTCGCGATGATTGTTGTCGCACAGTTCGCGGCCACGCTCACCGACGACACCG
GCGCTCCGCTGCTGTTGGTCAAAGGCGGATCGTCGCTGGAACTGCGCCGGGGAATTCCCGATTCGCGGACCTCCAAAGAC
TTCGACACGGTCGCACGTCGCGATATCGAATTAATCCATGAACAGCTCGCTGACGCGGGCGAGACGGGGTGGGAAGGATT
CACTGCAATCTTCACCGCCCCCGAAGAAATCGATGTTCCTGGTATGCCGGTCAAGCCGCGCCGATTCACCGCCAAGCTGA
GCTACCGAGGCCGGGCTTTCGCAACTGTTCCGATCGAGGTCTCCTCCGTCGAAGCCGGCAATGCCGACCAATTCGACACC
CTCACCTCAGACGCGCTCGGCCTCGTGGGCGTACCCGCAGCAGTCGCCGTACCCTGCATGACCATTCCCTGGCAAATCGC
GCAGAAGCTGCACGCAGTAACTGCCGTGCTCGAAGAACCGAAGGTCAACGACCGCGCTCACGACCTGGTGGACTTGCAGC
TTCTTGAAGGACTGTTGCTCGATGCCGACCTCATGCCGACGCGCAGCGCGTGCATCGCGATATTCGAAGCGCGCGCCCAG
CATCCTTGGCCACCGAGAGTCGCCACGCTGCCGCACTGGCCGCTGATCTATGCAGGTGCGCTGGAGGGGCTTGACCACCT
TGAACTCGCCAGGACGGTCGACGCGGCGGCCCAGGCAGTGCAGCGATTCGTTGCGCGGATTGATCGGGCGACGAAAAGAT
GA

Antitoxin


Download         Length: 208 a.a.        Molecular weight: 22531.68 Da        Isoelectric Point: 10.0518

>AT10016 NP_215560.1 NC_000962:1167053-1167676 [Mycobacterium tuberculosis H37Rv]
MCAKPYLIDTIAHMAIWDRLVEVAAEQHGYVTTRDARDIGVDPVQLRLLAGRGRLERVGRGVYRVPVLPRGEHDDLAAAV
SWTLGRGVISHESALALHALADVNPSRIHLTVPRNNHPRAAGGELYRVHRRDLQAAHVTSVDGIPVTTVARTIKDCVKTG
TDPYQLRAAIERAEAEGTLRRGSAAELRAALDETTAGLRARPKRASA

Download         Length: 624 bp

>AT10016 NC_000962:1167053-1167676 [Mycobacterium tuberculosis H37Rv]
TTGTGTGCAAAACCGTATCTAATTGATACGATTGCGCACATGGCTATCTGGGATCGCCTCGTCGAGGTTGCCGCCGAGCA
ACATGGCTACGTCACGACTCGCGATGCGCGAGACATCGGCGTCGACCCTGTGCAGCTCCGCCTCCTAGCGGGGCGCGGAC
GTCTTGAGCGTGTCGGCCGAGGTGTGTACCGGGTGCCCGTGCTGCCGCGTGGTGAGCACGACGATCTCGCAGCCGCAGTG
TCGTGGACTTTGGGGCGTGGCGTTATCTCGCATGAGTCGGCCTTGGCGCTTCATGCCCTCGCTGACGTGAACCCGTCGCG
CATCCATCTCACCGTCCCGCGCAACAACCATCCGCGTGCGGCCGGGGGCGAGCTGTACCGAGTTCACCGCCGCGACCTCC
AGGCAGCCCACGTCACTTCGGTCGACGGAATACCCGTCACGACGGTTGCGCGCACCATCAAAGACTGCGTGAAGACGGGC
ACGGATCCTTATCAGCTTCGGGCCGCGATCGAGCGAGCCGAAGCCGAGGGCACGCTTCGTCGTGGGTCAGCAGCTGAGCT
ACGCGCTGCGCTCGATGAGACCACTGCCGGATTACGCGCTCGGCCGAAGCGAGCATCGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6J7P
PDB 6J7Q
PDB 6Y5U
PDB 6J7O
PDB 6J7S
PDB 6J7T
PDB 7C46
PDB 6J7N
PDB 7C48
PDB 6J7R

Antitoxin

Source ID Structure
AlphaFold DB P96357

References

(1) Xia Yu et al. (2020) Characterization of a toxin-antitoxin system in Mycobacterium tuberculosis suggests neutralization by phosphorylation as the antitoxicity mechanism. Communications Biology 3(1):216. [PubMed:32382148] experimental

(2) Yiming Cai et al. (2020) A nucleotidyltransferase toxin inhibits growth of Mycobacterium tuberculosis through inactivation of tRNA acceptor stems. Science Advances 6(31):eabb6651. [PubMed:32923609] experimental


experimental experimental literature