Detailed information of TA system TA024064

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): TglT-TakA/AbiEii-AbiEi
• Location: 3243413..3244914
• Replicon: chromosome
• Accession: NC_012943
• Organism: Mycobacterium tuberculosis KZN 1435

Toxin (Protein)

• Gene name: tglT
• Uniprot ID: L7N4P5
• Locus tag: TBMG_RS15310
• Protein ID: WP_003898703.1
• Coordinates: 3243413..3244294 (-)
• Length: 294 a.a.

Antitoxin (Protein)

• Gene name: takA
• Uniprot ID: L7N5C7
• Locus tag: TBMG_RS15315
• Protein ID: WP_003405517.1
• Coordinates: 3244291..3244914 (-)
• Length: 208 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBMG_RS15285	3239134..3239580	-	447	WP_003405595.1	oxidation-sensing regulator MosR	-
TBMG_RS15290	3239653..3239856	+	204	WP_003405585.1	hypothetical protein	-
TBMG_RS15295	3239813..3240928	+	1116	WP_003405582.1	hypothetical protein	-
TBMG_RS15300	3241296..3242543	-	1248	WP_003900260.1	IS256-like element IS1081 family transposase	-
TBMG_RS15310	3243413..3244294	-	882	WP_003898703.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
TBMG_RS15315	3244291..3244914	-	624	WP_003405517.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
TBMG_RS15320	3245161..3246186	+	1026	WP_003405514.1	serine protease	-
TBMG_RS21595	3246469..3247396	+	928	Protein_3002	IS5-like element ISMt1 family transposase	-
TBMG_RS15335	3247565..3247804	+	240	WP_009936438.1	hypothetical protein	-
TBMG_RS15340	3248592..3249419	+	828	WP_003405370.1	PE domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_000962 Mycobacterium tuberculosis H37Rv IScluster/Tn TA010016 1158918..1170670 0.001	inside	IScluster/Tn	-	esxN	3241296..3253050	11754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (49-279)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (18-66)

Sequences

Toxin

Protein

• Length: 294 a.a.
• Molecular weight: 31958.65 Da
• Isoelectric Point: 6.3901

>T24064 WP_003898703.1 NC_012943:c3244294-3243413 [Mycobacterium tuberculosis KZN 1435]
VTKPYSSPPTNLRSLRDRLTQVAERQGVVFGRLQRHVAMIVVAQFAATLTDDTGAPLLLVKGGSSLELRRGIPDSRTSKD
FDTVARRDIELIHEQLADAGETGWEGFTAIFTAPEEIDVPGMPVKPRRFTAKLSYRGRAFATVPIEVSSVEAGNADQFDT
LTSDALGLVGVPAAVAVPCMTIPWQIAQKLHAVTAVLEEPKVNDRAHDLVDLQLLEGLLLDADLMPTRSACIAIFEARAQ
HPWPPRVATLPHWPLIYAGALEGLDHLELARTVDAAAQAVQRFVARIDRATKR

Nucleotide

• Length: 882 bp

>T24064 NC_012943:c3244294-3243413 [Mycobacterium tuberculosis KZN 1435]
GTGACCAAGCCCTATTCGTCGCCGCCAACGAACCTGCGCTCACTACGAGATCGGCTCACCCAAGTAGCGGAACGGCAAGG
TGTCGTGTTCGGTCGACTGCAGCGGCATGTCGCGATGATTGTTGTCGCACAGTTCGCGGCCACGCTCACCGACGACACCG
GCGCTCCGCTGCTGTTGGTCAAAGGCGGATCGTCGCTGGAACTGCGCCGGGGAATTCCCGATTCGCGGACCTCCAAAGAC
TTCGACACGGTCGCACGTCGCGATATCGAATTAATCCATGAACAGCTCGCTGACGCGGGCGAGACGGGGTGGGAAGGATT
CACTGCAATCTTCACCGCCCCCGAAGAAATCGATGTTCCTGGTATGCCGGTCAAGCCGCGCCGATTCACCGCCAAGCTGA
GCTACCGAGGCCGGGCTTTCGCAACTGTTCCGATCGAGGTCTCCTCCGTCGAAGCCGGCAATGCCGACCAATTCGACACC
CTCACCTCAGACGCGCTCGGCCTCGTGGGCGTACCCGCAGCAGTCGCCGTACCCTGCATGACCATTCCCTGGCAAATCGC
GCAGAAGCTGCACGCAGTAACTGCCGTGCTCGAAGAACCGAAGGTCAACGACCGCGCTCACGACCTGGTGGACTTGCAGC
TTCTTGAAGGACTGTTGCTCGATGCCGACCTCATGCCGACGCGCAGCGCGTGCATCGCGATATTCGAAGCGCGCGCCCAG
CATCCTTGGCCACCGAGAGTCGCCACGCTGCCGCACTGGCCGCTGATCTATGCAGGTGCGCTGGAGGGGCTTGACCACCT
TGAACTCGCCAGGACGGTCGACGCGGCGGCCCAGGCAGTGCAGCGATTCGTTGCGCGGATTGATCGGGCGACGAAAAGAT
GA

Antitoxin

Protein

• Length: 208 a.a.
• Molecular weight: 22531.68 Da
• Isoelectric Point: 10.0518

>AT24064 WP_003405517.1 NC_012943:c3244914-3244291 [Mycobacterium tuberculosis KZN 1435]
MCAKPYLIDTIAHMAIWDRLVEVAAEQHGYVTTRDARDIGVDPVQLRLLAGRGRLERVGRGVYRVPVLPRGEHDDLAAAV
SWTLGRGVISHESALALHALADVNPSRIHLTVPRNNHPRAAGGELYRVHRRDLQAAHVTSVDGIPVTTVARTIKDCVKTG
TDPYQLRAAIERAEAEGTLRRGSAAELRAALDETTAGLRARPKRASA

Nucleotide

• Length: 624 bp

>AT24064 NC_012943:c3244914-3244291 [Mycobacterium tuberculosis KZN 1435]
TTGTGTGCAAAACCGTATCTAATTGATACGATTGCGCACATGGCTATCTGGGATCGCCTCGTCGAGGTTGCCGCCGAGCA
ACATGGCTACGTCACGACTCGCGATGCGCGAGACATCGGCGTCGACCCTGTGCAGCTCCGCCTCCTAGCGGGGCGCGGAC
GTCTTGAGCGTGTCGGCCGAGGTGTGTACCGGGTGCCCGTGCTGCCGCGTGGTGAGCACGACGATCTCGCAGCCGCAGTG
TCGTGGACTTTGGGGCGTGGCGTTATCTCGCATGAGTCGGCCTTGGCGCTTCATGCCCTCGCTGACGTGAACCCGTCGCG
CATCCATCTCACCGTCCCGCGCAACAACCATCCGCGTGCGGCCGGGGGCGAGCTGTACCGAGTTCACCGCCGCGACCTCC
AGGCAGCCCACGTCACTTCGGTCGACGGAATACCCGTCACGACGGTTGCGCGCACCATCAAAGACTGCGTGAAGACGGGC
ACGGATCCTTATCAGCTTCGGGCCGCGATCGAGCGAGCCGAAGCCGAGGGCACGCTTCGTCGTGGGTCAGCAGCTGAGCT
ACGCGCTGCGCTCGATGAGACCACTGCCGGATTACGCGCTCGGCCGAAGCGAGCATCGGCGTGA

Structures

Toxin

• PDB: 7C46
• PDB: 6J7S
• PDB: 6J7T
• PDB: 7C48
• PDB: 6J7P
• PDB: 6J7Q
• PDB: 6Y5U
• PDB: 6J7O
• PDB: 6J7R
• PDB: 6J7N

Antitoxin

• AlphaFold DB: A0A7U4BT71