Detailed information of TA system TA189175

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): TglT-TakA/AbiEii-AbiEi
• Location: 1171144..1172645
• Replicon: chromosome
• Accession: NZ_CP069067
• Organism: Mycobacterium tuberculosis strain N1268

Toxin (Protein)

• Gene name: tglT
• Uniprot ID: L7N4P5
• Locus tag: JN146_RS05605
• Protein ID: WP_003898703.1
• Coordinates: 1171764..1172645 (+)
• Length: 294 a.a.

Antitoxin (Protein)

• Gene name: takA
• Uniprot ID: L7N5C7
• Locus tag: JN146_RS05600
• Protein ID: WP_003405517.1
• Coordinates: 1171144..1171767 (+)
• Length: 208 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN146_RS05580	1166640..1167467	-	828	WP_003405370.1	PE domain-containing protein	-
JN146_RS05585	1168255..1168494	-	240	WP_003405375.1	hypothetical protein	-
JN146_RS05590	1168663..1169590	-	928	Protein_1102	IS5-like element ISMt1 family transposase	-
JN146_RS05595	1169872..1170897	-	1026	WP_003405514.1	serine protease	-
JN146_RS05600	1171144..1171767	+	624	WP_003405517.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
JN146_RS05605	1171764..1172645	+	882	WP_003898703.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
JN146_RS05615	1174874..1176121	+	1248	WP_003901078.1	IS256-like element IS1081 family transposase	-
JN146_RS05620	1176489..1177604	-	1116	WP_003405582.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_000962 Mycobacterium tuberculosis H37Rv IScluster/Tn TA010016 1158918..1170670 0.003	inside	IScluster/Tn	-	esxN	1163009..1176121	13112

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (49-279)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (18-66)

Sequences

Toxin

Protein

• Length: 294 a.a.
• Molecular weight: 31958.65 Da
• Isoelectric Point: 6.3901

>T189175 WP_003898703.1 NZ_CP069067:1171764-1172645 [Mycobacterium tuberculosis]
VTKPYSSPPTNLRSLRDRLTQVAERQGVVFGRLQRHVAMIVVAQFAATLTDDTGAPLLLVKGGSSLELRRGIPDSRTSKD
FDTVARRDIELIHEQLADAGETGWEGFTAIFTAPEEIDVPGMPVKPRRFTAKLSYRGRAFATVPIEVSSVEAGNADQFDT
LTSDALGLVGVPAAVAVPCMTIPWQIAQKLHAVTAVLEEPKVNDRAHDLVDLQLLEGLLLDADLMPTRSACIAIFEARAQ
HPWPPRVATLPHWPLIYAGALEGLDHLELARTVDAAAQAVQRFVARIDRATKR

Nucleotide

• Length: 882 bp

>T189175 NZ_CP090681:c1616063-1615608 [Burkholderia multivorans]
ATGATCCTTGTTGATACGAACGTCATTTCCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
ACAGAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGGTGTCGCGGCGCTGCCGGAAGGGC
GCCGGCGGGACTGGCTGGCGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCCGCGAGCAAGGCCTATGCGCGCATTCGCGCGCACGCCCGTGCCGGCGGCAACGCGATCGCGCCCGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCCGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCGTGGGCGTCGCAGGGCGCGCCGGTGCGCCAGGGCATCGCGTCGAAATGA

Antitoxin

Protein

• Length: 208 a.a.
• Molecular weight: 22531.68 Da
• Isoelectric Point: 10.0518

>AT189175 WP_003405517.1 NZ_CP069067:1171144-1171767 [Mycobacterium tuberculosis]
MCAKPYLIDTIAHMAIWDRLVEVAAEQHGYVTTRDARDIGVDPVQLRLLAGRGRLERVGRGVYRVPVLPRGEHDDLAAAV
SWTLGRGVISHESALALHALADVNPSRIHLTVPRNNHPRAAGGELYRVHRRDLQAAHVTSVDGIPVTTVARTIKDCVKTG
TDPYQLRAAIERAEAEGTLRRGSAAELRAALDETTAGLRARPKRASA

Nucleotide

• Length: 624 bp

>AT189175 NZ_CP090681:c1616299-1616060 [Burkholderia multivorans]
ATGCCTGTGATTACCGTTCGAAATTTGCCGGATGAAGTGCATCGCGCCCTTCGGGTCCGCGCGGCACTGCACGGGCGCAG
TACCGAGGCAGAGGTGCGCGACATCCTCGAGCAGGCCGTGTTGTCGGAAGGGCGTGTGAAGCTGGGAACCTTGCTGGCGG
AAATCGGACGGGAGGCAGGCGGTGTCGATTTCGACATTCAGCGTGACAAGACGCCAACCGATCCGATGAGCTTCGAATGA

Structures

Toxin

• PDB: 7C46
• PDB: 6J7S
• PDB: 6J7T
• PDB: 7C48
• PDB: 6J7P
• PDB: 6J7Q
• PDB: 6Y5U
• PDB: 6J7O
• PDB: 6J7R
• PDB: 6J7N

Antitoxin

• AlphaFold DB: A0A7U4BT71