Detailed information of TA system TA139738

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): TglT-TakA/AbiEii-AbiEi
• Location: 3243262..3244763
• Replicon: chromosome
• Accession: NZ_CP043995
• Organism: Mycobacterium tuberculosis strain 4-0041P6C4

Toxin (Protein)

• Gene name: tglT
• Uniprot ID: L7N4P5
• Locus tag: FPJ87_RS15195
• Protein ID: WP_003898703.1
• Coordinates: 3243262..3244143 (-)
• Length: 294 a.a.

Antitoxin (Protein)

• Gene name: takA
• Uniprot ID: L7N5C7
• Locus tag: FPJ87_RS15200
• Protein ID: WP_003405517.1
• Coordinates: 3244140..3244763 (-)
• Length: 208 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ87_RS15175	3238983..3239429	-	447	WP_003405595.1	oxidation-sensing regulator MosR	-
FPJ87_RS15180	3239502..3239705	+	204	WP_003405585.1	hypothetical protein	-
FPJ87_RS15185	3239662..3240777	+	1116	WP_003405582.1	hypothetical protein	-
FPJ87_RS15190	3241145..3242392	-	1248	WP_003900260.1	IS256-like element IS1081 family transposase	-
FPJ87_RS15195	3243262..3244143	-	882	WP_003898703.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
FPJ87_RS15200	3244140..3244763	-	624	WP_003405517.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
FPJ87_RS15205	3245010..3246035	+	1026	WP_003405514.1	serine protease	-
FPJ87_RS15210	3246318..3247245	+	928	Protein_3002	IS5-like element ISMt1 family transposase	-
FPJ87_RS15215	3247414..3247653	+	240	WP_009936438.1	hypothetical protein	-
FPJ87_RS15220	3248441..3249268	+	828	WP_003405370.1	PE domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_000962 Mycobacterium tuberculosis H37Rv IScluster/Tn TA010016 1158918..1170670 0.001	inside	IScluster/Tn	-	esxN	3241145..3252899	11754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (49-279)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (18-66)

Sequences

Toxin

Protein

• Length: 294 a.a.
• Molecular weight: 31958.65 Da
• Isoelectric Point: 6.3901

>T139738 WP_003898703.1 NZ_CP043995:c3244143-3243262 [Mycobacterium tuberculosis]
VTKPYSSPPTNLRSLRDRLTQVAERQGVVFGRLQRHVAMIVVAQFAATLTDDTGAPLLLVKGGSSLELRRGIPDSRTSKD
FDTVARRDIELIHEQLADAGETGWEGFTAIFTAPEEIDVPGMPVKPRRFTAKLSYRGRAFATVPIEVSSVEAGNADQFDT
LTSDALGLVGVPAAVAVPCMTIPWQIAQKLHAVTAVLEEPKVNDRAHDLVDLQLLEGLLLDADLMPTRSACIAIFEARAQ
HPWPPRVATLPHWPLIYAGALEGLDHLELARTVDAAAQAVQRFVARIDRATKR

Nucleotide

• Length: 882 bp

>T139738 NZ_CP058790:c4084167-4083229 [Shigella flexneri]
ATGGATAAAGGCAGCCCATATTACAAACAGGTTTCCTTGCTCATAAGAATGCTTCCTGTGGTAGCCACAGAGACGGTTTT
TGCACTTAAAGGTGGCACCGCCATTAATTTATTTGTGAGAGATTTTCCTCGGTTATCTGTAGATATTGATCTTGCTTATC
TTCCACTTGAACCAAGAGATGAGGCTTTGGTTAATGTCAGGGCTGCATTGCAGCGCATTACAGACAGAATCAATACTCAA
CCAGATATCCGAGCGGCATTTCAGGATAATAAAGCTGATGAACTGAGAATCGTTGTATCAAGTCTGGTTGCGACAATCAA
AATTGAAGTGTCGCCCGTCGCCAGAGGTACATTGCATAGTGCAGAGATAATGCCAGTTCAAGAGTCTGTTGAAGACGAGT
TTGGTTATGCAGAGATTCAGGTAGTCAGTCTTCCCGATCTGTATGGTGGGAAATTGTGTGCTGCAATGGATCGCCAACAT
CCTCGCGACTTATTTGATGTCCGTATGTTACTTGGCAGTGAAGGGATTTCGAGAGAGATTTTAGTGGGCTTTTTAACCTA
TACACTAAGTCATCCTCGGCCTATTAATGAAGTTATGTCGCCAAACTGGCAGCCTCTGAATGAGAAATTTCAGGCAGAAT
TTGACGGAATGACTTTTGAAAAAGTTGAATGCGAAGACTTAGCCTCTGTTAGGCCTATGATGTTAACTTCGCTGCAAAAA
CATTTCACAGAAAGGGATCATGCTTTCCTTATGTCATTTAAAAGAGGGCAACCTGACTGGGCATTGTTTGATTATCCTAA
TGCAGCAGACTTGCCAGCGATTCGTTGGAAGTTGCAGAACATTTACAAATTGGCAAAGAATCAAGCAAAACATCAAGAGC
AATTAGATAAATTGAAGCAAGTGCTTGATGATTGGCTTGTTAACGCAAACGCCGAGTAA

Antitoxin

Protein

• Length: 208 a.a.
• Molecular weight: 22531.68 Da
• Isoelectric Point: 10.0518

>AT139738 WP_003405517.1 NZ_CP043995:c3244763-3244140 [Mycobacterium tuberculosis]
MCAKPYLIDTIAHMAIWDRLVEVAAEQHGYVTTRDARDIGVDPVQLRLLAGRGRLERVGRGVYRVPVLPRGEHDDLAAAV
SWTLGRGVISHESALALHALADVNPSRIHLTVPRNNHPRAAGGELYRVHRRDLQAAHVTSVDGIPVTTVARTIKDCVKTG
TDPYQLRAAIERAEAEGTLRRGSAAELRAALDETTAGLRARPKRASA

Nucleotide

• Length: 624 bp

>AT139738 NZ_CP058790:c4084951-4084160 [Shigella flexneri]
ATGTCATCTAAGATAAACTGGCTTGTTGCTCATACTTCTCCTGGAGCGCTAGTACTTCAGCAATGGCTGACTGAAAACGG
CGTGAGCTACTCGTTGGCTCAGAAGTACGCGCAGAACGGTTGGCTGAAGAAGCTTAGTTCTGGTGTGTACTATCGTCCAA
ATGCGCAAGGCGATATAAAGCCAACTTGGGTTGATGCCATTCAAGCATTAGATGTGCAATTGGGCGTTTCAGTTCATTTG
GCTGGATTGAGCAGCTTAACTCACCAAGGACTGAGTCACTATTTACAGCTGAACAAAGAGCAAGTTTGGATTTGCGTTAA
AAACAAGTCGTCCTTACCAAAATGGTTTCGTGAATTCCCTTATCAGAATTGGTTTTACTGTGGAAACCATAAGCTTGAAC
TGAATCCCGAGAAAGATTTGAAAAGGATCACGGTTAAAGAGAAAGAGCTCACTGTCAGCTGTGCAGAACTTGCTGCCTAT
GAAGTGGTAGACGGGATTGGGAAGCTGATTTCATTTGAGCATGTCGCAGAATTATTTCAGGGTTTAGTCAATCTTAGCCC
TAGAAAAGTACAAGATATTCTTGAACGAAGCAGCTCTGTTCAGGCAAACCGAATATTTCTATTTCTGGGTCGATACTACG
ATCACCAGTGGATCAATCGCATAGATGAAACAAGAATTAAATTGGGTGCAGGAAAGCGGCAGGTTGTCGAAAAAGGACGT
TTTGATGAGCGATATCAAATCACAGTGCCAGAGATATTAAGCGTCAAAAAAGGTGAACAACACAATGGATAA

Structures

Toxin

• PDB: 7C46
• PDB: 6J7S
• PDB: 6J7T
• PDB: 7C48
• PDB: 6J7P
• PDB: 6J7Q
• PDB: 6Y5U
• PDB: 6J7O
• PDB: 6J7R
• PDB: 6J7N

Antitoxin

• AlphaFold DB: A0A7U4BT71