Detailed information of TA system TA025450

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): TglT-TakA/AbiEii-AbiEi
• Location: 1163449..1164950
• Replicon: chromosome
• Accession: NC_015758
• Organism: Mycobacterium africanum GM041182

Toxin (Protein)

• Gene name: tglT
• Uniprot ID: L7N4P5
• Locus tag: MAF_RS05530
• Protein ID: WP_003898703.1
• Coordinates: 1164069..1164950 (+)
• Length: 294 a.a.

Antitoxin (Protein)

• Gene name: takA
• Uniprot ID: L7N5C7
• Locus tag: MAF_RS05525
• Protein ID: WP_003405517.1
• Coordinates: 1163449..1164072 (+)
• Length: 208 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAF_RS05500	1158944..1159771	-	828	WP_003405370.1	PE domain-containing protein	-
MAF_RS05505	1160559..1160798	-	240	WP_003405375.1	hypothetical protein	-
MAF_RS20810	1160967..1161894	-	928	Protein_1078	IS5-like element ISMt1 family transposase	-
MAF_RS05520	1162177..1163202	-	1026	WP_003405514.1	serine protease	-
MAF_RS05525	1163449..1164072	+	624	WP_003405517.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
MAF_RS05530	1164069..1164950	+	882	WP_003898703.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
MAF_RS05540	1165820..1167067	+	1248	WP_003901078.1	IS256-like element IS1081 family transposase	-
MAF_RS05545	1167435..1168550	-	1116	WP_003405582.1	hypothetical protein	-
MAF_RS05550	1168507..1168710	-	204	WP_003405585.1	hypothetical protein	-
MAF_RS05555	1168783..1169229	+	447	WP_003405595.1	oxidation-sensing regulator MosR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_000962 Mycobacterium tuberculosis H37Rv IScluster/Tn TA010016 1158918..1170670 0.001	inside	IScluster/Tn	-	esxN	1155313..1167067	11754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (49-279)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (18-66)

Sequences

Toxin

Protein

• Length: 294 a.a.
• Molecular weight: 31958.65 Da
• Isoelectric Point: 6.3901

>T25450 WP_003898703.1 NC_015758:1164069-1164950 [Mycobacterium tuberculosis variant africanum GM041182]
VTKPYSSPPTNLRSLRDRLTQVAERQGVVFGRLQRHVAMIVVAQFAATLTDDTGAPLLLVKGGSSLELRRGIPDSRTSKD
FDTVARRDIELIHEQLADAGETGWEGFTAIFTAPEEIDVPGMPVKPRRFTAKLSYRGRAFATVPIEVSSVEAGNADQFDT
LTSDALGLVGVPAAVAVPCMTIPWQIAQKLHAVTAVLEEPKVNDRAHDLVDLQLLEGLLLDADLMPTRSACIAIFEARAQ
HPWPPRVATLPHWPLIYAGALEGLDHLELARTVDAAAQAVQRFVARIDRATKR

Nucleotide

• Length: 882 bp

>T25450 NC_015758:1164069-1164950 [Mycobacterium tuberculosis variant africanum GM041182]
GTGACCAAGCCCTATTCGTCGCCGCCAACGAACCTGCGCTCACTACGAGATCGGCTCACCCAAGTAGCGGAACGGCAAGG
TGTCGTGTTCGGTCGACTGCAGCGGCATGTCGCGATGATTGTTGTCGCACAGTTCGCGGCCACGCTCACCGACGACACCG
GCGCTCCGCTGCTGTTGGTCAAAGGCGGATCGTCGCTGGAACTGCGCCGGGGAATTCCCGATTCGCGGACCTCCAAAGAC
TTCGACACGGTCGCACGTCGCGATATCGAATTAATCCATGAACAGCTCGCTGACGCGGGCGAGACGGGGTGGGAAGGATT
CACTGCAATCTTCACCGCCCCCGAAGAAATCGATGTTCCTGGTATGCCGGTCAAGCCGCGCCGATTCACCGCCAAGCTGA
GCTACCGAGGCCGGGCTTTCGCAACTGTTCCGATCGAGGTCTCCTCCGTCGAAGCCGGCAATGCCGACCAATTCGACACC
CTCACCTCAGACGCGCTCGGCCTCGTGGGCGTACCCGCAGCAGTCGCCGTACCCTGCATGACCATTCCCTGGCAAATCGC
GCAGAAGCTGCACGCAGTAACTGCCGTGCTCGAAGAACCGAAGGTCAACGACCGCGCTCACGACCTGGTGGACTTGCAGC
TTCTTGAAGGACTGTTGCTCGATGCCGACCTCATGCCGACGCGCAGCGCGTGCATCGCGATATTCGAAGCGCGCGCCCAG
CATCCTTGGCCACCGAGAGTCGCCACGCTGCCGCACTGGCCGCTGATCTATGCAGGTGCGCTGGAGGGGCTTGACCACCT
TGAACTCGCCAGGACGGTCGACGCGGCGGCCCAGGCAGTGCAGCGATTCGTTGCGCGGATTGATCGGGCGACGAAAAGAT
GA

Antitoxin

Protein

• Length: 208 a.a.
• Molecular weight: 22531.68 Da
• Isoelectric Point: 10.0518

>AT25450 WP_003405517.1 NC_015758:1163449-1164072 [Mycobacterium tuberculosis variant africanum GM041182]
MCAKPYLIDTIAHMAIWDRLVEVAAEQHGYVTTRDARDIGVDPVQLRLLAGRGRLERVGRGVYRVPVLPRGEHDDLAAAV
SWTLGRGVISHESALALHALADVNPSRIHLTVPRNNHPRAAGGELYRVHRRDLQAAHVTSVDGIPVTTVARTIKDCVKTG
TDPYQLRAAIERAEAEGTLRRGSAAELRAALDETTAGLRARPKRASA

Nucleotide

• Length: 624 bp

>AT25450 NC_015758:1163449-1164072 [Mycobacterium tuberculosis variant africanum GM041182]
TTGTGTGCAAAACCGTATCTAATTGATACGATTGCGCACATGGCTATCTGGGATCGCCTCGTCGAGGTTGCCGCCGAGCA
ACATGGCTACGTCACGACTCGCGATGCGCGAGACATCGGCGTCGACCCTGTGCAGCTCCGCCTCCTAGCGGGGCGCGGAC
GTCTTGAGCGTGTCGGCCGAGGTGTGTACCGGGTGCCCGTGCTGCCGCGTGGTGAGCACGACGATCTCGCAGCCGCAGTG
TCGTGGACTTTGGGGCGTGGCGTTATCTCGCATGAGTCGGCCTTGGCGCTTCATGCCCTCGCTGACGTGAACCCGTCGCG
CATCCATCTCACCGTCCCGCGCAACAACCATCCGCGTGCGGCCGGGGGCGAGCTGTACCGAGTTCACCGCCGCGACCTCC
AGGCAGCCCACGTCACTTCGGTCGACGGAATACCCGTCACGACGGTTGCGCGCACCATCAAAGACTGCGTGAAGACGGGC
ACGGATCCTTATCAGCTTCGGGCCGCGATCGAGCGAGCCGAAGCCGAGGGCACGCTTCGTCGTGGGTCAGCAGCTGAGCT
ACGCGCTGCGCTCGATGAGACCACTGCCGGATTACGCGCTCGGCCGAAGCGAGCATCGGCGTGA

Structures

Toxin

• PDB: 7C46
• PDB: 6J7S
• PDB: 6J7T
• PDB: 7C48
• PDB: 6J7P
• PDB: 6J7Q
• PDB: 6Y5U
• PDB: 6J7O
• PDB: 6J7R
• PDB: 6J7N

Antitoxin

• AlphaFold DB: A0A7U4BT71