Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   J4H94_RS21570 Genome accession   NZ_CP071899
Coordinates   1006019..1006204 (-) Length   61 a.a.
NCBI ID   WP_133456153.1    Uniprot ID   -
Organism   Stutzerimonas frequens strain TPA3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1001019..1011204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4H94_RS04685 (J4H94_04685) - 1002793..1003689 (-) 897 WP_208217599.1 ABC transporter ATP-binding protein -
  J4H94_RS04690 (J4H94_04690) - 1003686..1004522 (-) 837 WP_166082509.1 hypothetical protein -
  J4H94_RS04695 (J4H94_04695) - 1004524..1005333 (-) 810 WP_166082500.1 ABC transporter permease subunit -
  J4H94_RS04700 (J4H94_04700) - 1005549..1006001 (-) 453 WP_133456154.1 hypothetical protein -
  J4H94_RS21570 (J4H94_04705) pilA 1006019..1006204 (-) 186 WP_133456153.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  J4H94_RS04710 (J4H94_04710) pilB 1006643..1008346 (+) 1704 WP_208217600.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J4H94_RS04715 (J4H94_04715) pilC 1008349..1009569 (+) 1221 WP_133456151.1 type II secretion system F family protein Machinery gene
  J4H94_RS04720 (J4H94_04720) pilD 1009573..1010439 (+) 867 WP_133456150.1 A24 family peptidase Machinery gene
  J4H94_RS04725 (J4H94_04725) coaE 1010573..1011181 (+) 609 WP_208217601.1 dephospho-CoA kinase -

Sequence


Protein


Download         Length: 61 a.a.        Molecular weight: 6614.00 Da        Isoelectric Point: 10.4849

>NTDB_id=548909 J4H94_RS21570 WP_133456153.1 1006019..1006204(-) (pilA) [Stutzerimonas frequens strain TPA3]
MKSIKKQKGFTLIELMIVVAIIGILAAIAIPQFNEYRAKANDSSAKADTKNMITVMSAAMI

Nucleotide


Download         Length: 186 bp        

>NTDB_id=548909 J4H94_RS21570 WP_133456153.1 1006019..1006204(-) (pilA) [Stutzerimonas frequens strain TPA3]
ATGAAATCCATCAAGAAGCAAAAAGGTTTTACCTTGATTGAACTGATGATCGTGGTTGCGATCATCGGCATTCTAGCGGC
CATTGCCATTCCGCAGTTCAACGAGTATCGCGCTAAGGCGAACGACTCTTCGGCCAAAGCTGACACAAAGAACATGATCA
CCGTCATGTCCGCGGCAATGATCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

59.649

93.443

0.557

  pilA/pilA1 Eikenella corrodens VA1

55.556

88.525

0.492

  comP Acinetobacter baylyi ADP1

58.824

83.607

0.492

  pilA Vibrio parahaemolyticus RIMD 2210633

60

81.967

0.492

  pilA Acinetobacter nosocomialis M2

76.923

63.934

0.492

  pilA2 Legionella pneumophila strain ERS1305867

61.224

80.328

0.492

  pilA2 Legionella pneumophila str. Paris

61.224

80.328

0.492

  pilA Acinetobacter baumannii strain A118

85.294

55.738

0.475

  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

55.769

85.246

0.475

  pilA Ralstonia pseudosolanacearum GMI1000

64.444

73.77

0.475

  pilA/pilAI Pseudomonas stutzeri DSM 10701

66.667

68.852

0.459

  pilA Vibrio campbellii strain DS40M4

77.778

59.016

0.459

  pilA/pilAII Pseudomonas stutzeri DSM 10701

57.143

80.328

0.459

  pilA Vibrio cholerae C6706

75

59.016

0.443

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

75

59.016

0.443

  pilA Vibrio cholerae strain A1552

75

59.016

0.443

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

66.667

63.934

0.426

  pilE Neisseria gonorrhoeae strain FA1090

66.667

63.934

0.426

  pilE Neisseria gonorrhoeae MS11

66.667

63.934

0.426

  comF Acinetobacter baylyi ADP1

55.556

73.77

0.41

  pilA Haemophilus influenzae Rd KW20

70.588

55.738

0.393

  pilA Haemophilus influenzae 86-028NP

70.588

55.738

0.393

  pilA Glaesserella parasuis strain SC1401

69.697

54.098

0.377

  pilA/pilA4 Thermus thermophilus HB27

57.5

65.574

0.377

  pilX Neisseria meningitidis 8013

60.526

62.295

0.377

  pilL Neisseria gonorrhoeae MS11

60.526

62.295

0.377

  pilA/pilA1 Synechocystis sp. PCC 6803

53.488

70.492

0.377

  fimU Acinetobacter baumannii D1279779

36.667

98.361

0.361

  comE Acinetobacter baylyi ADP1

59.459

60.656

0.361