Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J4H94_RS04710 Genome accession   NZ_CP071899
Coordinates   1006643..1008346 (+) Length   567 a.a.
NCBI ID   WP_208217600.1    Uniprot ID   -
Organism   Stutzerimonas frequens strain TPA3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1001643..1013346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4H94_RS04685 (J4H94_04685) - 1002793..1003689 (-) 897 WP_208217599.1 ABC transporter ATP-binding protein -
  J4H94_RS04690 (J4H94_04690) - 1003686..1004522 (-) 837 WP_166082509.1 hypothetical protein -
  J4H94_RS04695 (J4H94_04695) - 1004524..1005333 (-) 810 WP_166082500.1 ABC transporter permease subunit -
  J4H94_RS04700 (J4H94_04700) - 1005549..1006001 (-) 453 WP_133456154.1 hypothetical protein -
  J4H94_RS21570 (J4H94_04705) pilA 1006019..1006204 (-) 186 WP_133456153.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  J4H94_RS04710 (J4H94_04710) pilB 1006643..1008346 (+) 1704 WP_208217600.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J4H94_RS04715 (J4H94_04715) pilC 1008349..1009569 (+) 1221 WP_133456151.1 type II secretion system F family protein Machinery gene
  J4H94_RS04720 (J4H94_04720) pilD 1009573..1010439 (+) 867 WP_133456150.1 A24 family peptidase Machinery gene
  J4H94_RS04725 (J4H94_04725) coaE 1010573..1011181 (+) 609 WP_208217601.1 dephospho-CoA kinase -
  J4H94_RS04730 (J4H94_04730) yacG 1011178..1011375 (+) 198 WP_102840588.1 DNA gyrase inhibitor YacG -
  J4H94_RS04735 (J4H94_04735) - 1011400..1012080 (-) 681 WP_102840589.1 energy-coupling factor ABC transporter permease -
  J4H94_RS04740 (J4H94_04740) - 1012119..1013155 (-) 1037 Protein_932 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62129.02 Da        Isoelectric Point: 5.3342

>NTDB_id=548885 J4H94_RS04710 WP_208217600.1 1006643..1008346(+) (pilB) [Stutzerimonas frequens strain TPA3]
MNESAPLGGLARQLVLTGQLDEKIAQQAQQQALRNQVPLVTWLVQNKLVKGRVLGDVAAEQFGVPFFDLGALDRDAQPKD
LVSEKLVRQHRALPLQRRGNKLYVAVSDPTNHQAISDIQFNTGLSVEAVLVEDDKLGEAIEKFFESATSSLEGLNDSELD
GLDIEVAGDAKEDDAAHNEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRFRTDGMLHEVARPPVQLVSRISAR
LKVMASLDIAERRKPQDGRIKMKVSSTKSIDFRVNTLPTLWGEKIVMRILDSSSAKMGIDALGYEDDQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPSCKQEVDVPRDVLLSEGFP
EARIGSFKLYGPVGCDSCKGGYKGRVGIYEVVKITPALQRLIMEEGNSIDIALQARKDGFNDLRTSGLVKAMNGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=548885 J4H94_RS04710 WP_208217600.1 1006643..1008346(+) (pilB) [Stutzerimonas frequens strain TPA3]
ATGAACGAGAGCGCTCCCCTCGGCGGCTTGGCCCGGCAGTTGGTTCTGACTGGTCAGCTTGATGAAAAGATTGCCCAGCA
AGCTCAGCAGCAGGCCTTGCGTAACCAAGTGCCACTAGTTACTTGGCTGGTTCAGAACAAGTTAGTCAAGGGACGCGTTC
TGGGTGATGTCGCGGCAGAGCAGTTCGGTGTGCCGTTCTTCGACTTGGGCGCGCTGGATAGGGATGCCCAGCCAAAGGAC
CTTGTCAGTGAGAAGCTGGTGCGTCAGCACCGTGCCTTGCCCTTACAGCGGCGCGGCAACAAGCTTTATGTGGCGGTTTC
CGATCCAACCAATCATCAGGCGATCAGTGATATTCAGTTCAATACGGGGCTTTCCGTTGAGGCTGTGCTGGTTGAGGACG
ACAAGCTCGGTGAGGCCATCGAAAAGTTCTTCGAGTCGGCGACCTCGTCCCTCGAAGGATTGAACGATTCCGAGTTGGAT
GGCTTGGATATCGAAGTGGCCGGTGACGCCAAGGAGGACGATGCGGCGCATAACGAAGCCGATGATGCGCCAGTGGTGCG
GTTTGTTAACAAGATGCTGCTCGATGCGATCAAGGGTGGTTCGTCCGATTTGCACTTCGAGCCATATGAAAAGAGCTATC
GGGTGCGCTTTCGTACGGACGGCATGTTGCACGAGGTTGCCCGCCCGCCGGTCCAGTTGGTCAGCCGTATCTCAGCGCGT
CTGAAAGTAATGGCCAGTCTGGATATCGCCGAGCGGCGCAAGCCGCAGGACGGGCGGATCAAGATGAAGGTCTCGAGCAC
CAAGTCGATCGATTTTCGCGTGAATACGCTACCCACCCTGTGGGGCGAGAAGATTGTTATGCGAATCTTGGATTCTTCCA
GTGCCAAGATGGGTATTGATGCGCTGGGGTATGAGGATGACCAGAAGGAGCTCTATCTAAGCGCGCTGAAGCAGCCGCAA
GGGATGATTCTGGTTACCGGCCCGACAGGCTCGGGCAAGACTGTCTCGCTTTATACCGGCCTGAATATTCTCAATACGGT
CGATGTGAATATCTCCACTGCGGAGGATCCGGTAGAGATCAATTTGGAAGGTATCAATCAGGTCAACGTAAATCCGAAGC
AGGGCATGGACTTTGCGCAGGCGCTTCGAGCGTTCCTGCGCCAGGATCCGGACATCATTATGGTGGGTGAGATTCGAGAT
CTCGAAACCGCCGAGATTGCCATCAAGGCAGCGCAGACCGGGCACATGGTGATGTCGACTCTGCATACCAATAGTGCTGC
GGAAACGCTTACGCGCTTGCGCAACATGGGGGTGCCGGCATTCAACATTGCCACATCAGTCAACCTGATCATTGCTCAGC
GGCTGGCTCGTAAGCTGTGCCCCAGCTGCAAACAAGAGGTCGATGTGCCGCGAGATGTTCTGCTCAGCGAAGGATTTCCT
GAAGCGCGAATCGGTTCCTTCAAGCTTTACGGGCCGGTGGGGTGCGATAGCTGCAAGGGAGGCTACAAGGGGCGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCGGCCCTGCAGCGGCTTATCATGGAAGAAGGCAATTCCATCGATATCGCCTTGC
AGGCGCGTAAAGATGGGTTCAATGACCTGCGCACTTCGGGTCTGGTCAAGGCCATGAACGGCGTCACCAGCCTGGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.649

100

0.6
  pilB Acinetobacter baylyi ADP1

58.511

99.471

0.582
  pilB Legionella pneumophila strain ERS1305867

54.895

100

0.554
  pilB Vibrio cholerae strain A1552

51.636

97.002

0.501
  pilF Neisseria gonorrhoeae MS11

51.197

95.767

0.49
  pilB Vibrio campbellii strain DS40M4

49.819

97.707

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

53.649

89.418

0.48
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.72

93.122

0.379
  pilF Thermus thermophilus HB27

37.852

100

0.379