Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   AAG685_RS23515 Genome accession   NZ_CP152345
Coordinates   5149855..5150040 (-) Length   61 a.a.
NCBI ID   WP_406665185.1    Uniprot ID   -
Organism   Pseudomonas indoloxydans strain SDU_PSEU1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 5144855..5155040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG685_RS23485 nadC 5145762..5146610 (+) 849 WP_406665173.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AAG685_RS23490 - 5146737..5147648 (-) 912 WP_406665175.1 ABC transporter ATP-binding protein -
  AAG685_RS23495 - 5147645..5148493 (-) 849 WP_406665177.1 hypothetical protein -
  AAG685_RS23500 - 5148490..5148702 (-) 213 WP_406665179.1 hypothetical protein -
  AAG685_RS23505 - 5148710..5149306 (-) 597 WP_406665181.1 ABC transporter permease -
  AAG685_RS23510 - 5149415..5149849 (-) 435 WP_406665183.1 hypothetical protein -
  AAG685_RS23515 pilA 5149855..5150040 (-) 186 WP_406665185.1 type IV pilin protein Machinery gene
  AAG685_RS23520 pilB 5150269..5151969 (+) 1701 WP_406665187.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAG685_RS23525 pilC 5151973..5153193 (+) 1221 WP_406665189.1 type II secretion system F family protein Machinery gene
  AAG685_RS23530 pilD 5153198..5154064 (+) 867 WP_406665191.1 prepilin peptidase Machinery gene
  AAG685_RS23535 coaE 5154266..5154874 (+) 609 WP_406665193.1 dephospho-CoA kinase -

Sequence


Protein


Download         Length: 61 a.a.        Molecular weight: 6649.91 Da        Isoelectric Point: 10.2806

>NTDB_id=988540 AAG685_RS23515 WP_406665185.1 5149855..5150040(-) (pilA) [Pseudomonas indoloxydans strain SDU_PSEU1]
MKSLKKQKGFTLIELMIVVAIIGILAAIAIPQFNEYRAKTNDSTAQADAKNVLTVMTVAQQ

Nucleotide


Download         Length: 186 bp        

>NTDB_id=988540 AAG685_RS23515 WP_406665185.1 5149855..5150040(-) (pilA) [Pseudomonas indoloxydans strain SDU_PSEU1]
ATGAAGTCTCTGAAGAAGCAAAAGGGTTTCACCCTGATCGAACTGATGATCGTCGTTGCGATCATCGGCATCCTGGCAGC
CATCGCCATTCCGCAGTTCAACGAGTATCGCGCCAAGACGAATGACTCCACTGCGCAGGCTGACGCCAAAAACGTGCTCA
CCGTAATGACCGTGGCTCAGCAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

60.714

91.803

0.557

  pilA Pseudomonas aeruginosa PAK

70.455

72.131

0.508

  pilA Acinetobacter nosocomialis M2

71.429

68.852

0.492

  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.604

86.885

0.492

  pilA2 Legionella pneumophila str. Paris

65.217

75.41

0.492

  pilA2 Legionella pneumophila strain ERS1305867

65.217

75.41

0.492

  pilA Acinetobacter baumannii strain A118

78.378

60.656

0.475

  pilA/pilAI Pseudomonas stutzeri DSM 10701

66.667

68.852

0.459

  pilA/pilA1 Eikenella corrodens VA1

75.676

60.656

0.459

  pilA Vibrio parahaemolyticus RIMD 2210633

56

81.967

0.459

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

62.222

73.77

0.459

  pilA Vibrio campbellii strain DS40M4

77.143

57.377

0.443

  pilA Vibrio cholerae C6706

75

59.016

0.443

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

75

59.016

0.443

  pilA Vibrio cholerae strain A1552

75

59.016

0.443

  pilA/pilAII Pseudomonas stutzeri DSM 10701

55.102

80.328

0.443

  comF Acinetobacter baylyi ADP1

57.778

73.77

0.426

  pilA/pilA1 Synechocystis sp. PCC 6803

43.86

93.443

0.41

  pilE Neisseria gonorrhoeae strain FA1090

64.103

63.934

0.41

  pilE Neisseria gonorrhoeae MS11

64.103

63.934

0.41

  pilE Acinetobacter baumannii D1279779

52.174

75.41

0.393

  comE Acinetobacter baylyi ADP1

54.545

72.131

0.393

  pilA Haemophilus influenzae 86-028NP

69.697

54.098

0.377

  pilA Haemophilus influenzae Rd KW20

69.697

54.098

0.377

  pilL Neisseria gonorrhoeae MS11

60.526

62.295

0.377

  pilA Glaesserella parasuis strain SC1401

68.75

52.459

0.361

  pilA/pilA4 Thermus thermophilus HB27

55

65.574

0.361

  pilE Legionella pneumophila strain ERS1305867

41.509

86.885

0.361

  pilE Legionella pneumophila str. Paris

41.509

86.885

0.361


Multiple sequence alignment