Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AAG685_RS23525 Genome accession   NZ_CP152345
Coordinates   5151973..5153193 (+) Length   406 a.a.
NCBI ID   WP_406665189.1    Uniprot ID   -
Organism   Pseudomonas indoloxydans strain SDU_PSEU1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5146973..5158193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG685_RS23495 - 5147645..5148493 (-) 849 WP_406665177.1 hypothetical protein -
  AAG685_RS23500 - 5148490..5148702 (-) 213 WP_406665179.1 hypothetical protein -
  AAG685_RS23505 - 5148710..5149306 (-) 597 WP_406665181.1 ABC transporter permease -
  AAG685_RS23510 - 5149415..5149849 (-) 435 WP_406665183.1 hypothetical protein -
  AAG685_RS23515 pilA 5149855..5150040 (-) 186 WP_406665185.1 type IV pilin protein Machinery gene
  AAG685_RS23520 pilB 5150269..5151969 (+) 1701 WP_406665187.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAG685_RS23525 pilC 5151973..5153193 (+) 1221 WP_406665189.1 type II secretion system F family protein Machinery gene
  AAG685_RS23530 pilD 5153198..5154064 (+) 867 WP_406665191.1 prepilin peptidase Machinery gene
  AAG685_RS23535 coaE 5154266..5154874 (+) 609 WP_406665193.1 dephospho-CoA kinase -
  AAG685_RS23540 yacG 5154871..5155065 (+) 195 WP_406665195.1 DNA gyrase inhibitor YacG -
  AAG685_RS23545 - 5155154..5155369 (-) 216 WP_406665197.1 hypothetical protein -
  AAG685_RS23550 - 5155474..5156163 (-) 690 WP_406665199.1 energy-coupling factor ABC transporter permease -
  AAG685_RS23555 - 5156392..5157021 (+) 630 WP_406665201.1 DUF1780 domain-containing protein -
  AAG685_RS23560 - 5157018..5157566 (+) 549 WP_406665203.1 MOSC domain-containing protein -
  AAG685_RS23565 - 5157591..5157764 (+) 174 WP_406665205.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44555.26 Da        Isoelectric Point: 9.5782

>NTDB_id=988542 AAG685_RS23525 WP_406665189.1 5151973..5153193(+) (pilC) [Pseudomonas indoloxydans strain SDU_PSEU1]
MAEKALKTSVFAWEGTDRKGAKIKGELSGQSPALVKAQLRKQGINPLKVRKKAISLFSAGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIIAEGFDNPNMRKLIDEVKQEVAAGNSFASSLRKKPEYFDDLYCNLVDSGEQSGALETLLDRIATYKEKT
EELKAKIKKAMTYPIAVIVVALIVTTILLIKVVPQFESVFEGFGAQLPAFTMMVVNMSRWLQEWWFLVLLVLFGSIYTFS
QTYKRSEKFRDSLDRALLKAPIVGDILYKAVVARFARTLATTFAAGVPLVDALDSVSGATGNVVFRHAVNRIKTDVSAGS
QLNFSMRNTGIFPTMAVQMTAIGEEAGSLDEMLDKTASYYEAEVDNAVDNLTTLMEPLIMSVLGVLVGGLIIAMYLPIFQ
LGAVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=988542 AAG685_RS23525 WP_406665189.1 5151973..5153193(+) (pilC) [Pseudomonas indoloxydans strain SDU_PSEU1]
ATGGCGGAAAAAGCTCTAAAAACCAGCGTCTTCGCCTGGGAAGGTACCGATAGAAAAGGTGCCAAGATCAAGGGTGAGCT
CTCTGGGCAGTCACCTGCGCTGGTCAAGGCGCAACTGCGCAAACAGGGCATCAATCCGCTCAAGGTGCGCAAGAAAGCGA
TCTCTCTGTTCAGTGCGGGCAAGAAGATCAAGCCGATGGACATCGCCCTGTTCACCCGCCAGATGGCCACCATGATGAAG
GCCGGGGTACCGCTTCTGCAGTCCTTCGACATCATCGCCGAGGGTTTCGACAACCCCAACATGCGCAAGCTGATCGACGA
GGTCAAACAGGAAGTGGCGGCCGGTAACAGCTTCGCCTCCTCGCTGCGCAAGAAGCCGGAATATTTCGACGACCTGTACT
GCAACCTGGTGGACTCGGGCGAGCAATCCGGTGCGCTGGAGACCCTGCTGGATCGGATCGCCACCTACAAGGAAAAGACC
GAAGAGCTGAAGGCCAAGATCAAGAAAGCCATGACCTATCCCATCGCGGTAATCGTGGTGGCGCTGATCGTGACGACCAT
CCTGCTGATCAAGGTGGTGCCGCAGTTCGAAAGCGTGTTCGAAGGCTTCGGCGCCCAGTTGCCGGCCTTCACCATGATGG
TGGTCAACATGTCGAGGTGGCTGCAGGAGTGGTGGTTCCTGGTACTGCTGGTTCTCTTCGGCTCCATCTATACCTTCAGC
CAGACCTACAAGCGCTCGGAGAAGTTTCGCGACTCGCTGGACAGGGCACTGCTCAAGGCCCCGATCGTAGGGGACATTCT
CTACAAGGCCGTGGTCGCGCGTTTCGCGCGCACACTGGCCACCACCTTCGCAGCAGGCGTGCCGCTGGTGGATGCGCTGG
ACTCGGTTTCCGGTGCAACCGGCAACGTGGTGTTTCGCCACGCGGTCAACCGGATCAAGACGGACGTGTCCGCCGGCTCC
CAGCTCAACTTCTCCATGCGCAACACCGGCATCTTCCCCACCATGGCCGTACAGATGACCGCCATCGGTGAAGAGGCGGG
TTCGCTCGATGAGATGCTGGACAAGACCGCAAGCTACTACGAGGCGGAAGTGGACAACGCGGTGGACAACCTGACCACCC
TGATGGAACCGCTCATCATGTCGGTGCTGGGCGTTCTGGTTGGCGGCCTGATCATTGCGATGTACCTGCCAATCTTCCAA
CTCGGCGCGGTGGTCGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

80

99.754

0.798
  pilC Acinetobacter baylyi ADP1

61.823

100

0.618
  pilC Acinetobacter baumannii D1279779

60.741

99.754

0.606
  pilC Legionella pneumophila strain ERS1305867

53.283

97.537

0.52
  pilG Neisseria gonorrhoeae MS11

45.025

99.015

0.446
  pilG Neisseria meningitidis 44/76-A

45.025

99.015

0.446
  pilC Vibrio cholerae strain A1552

41.379

100

0.414
  pilC Vibrio campbellii strain DS40M4

42.065

97.783

0.411

Multiple sequence alignment